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Espectroscopía vibracional para la caracterización rápida de la susceptibilidad a vancomicina en Enterococci

Ava Rossetti¹, Xenia Kostoulias^2,3,4, Magdalena Giergiel¹

¹School of Chemistry, Faculty of Science, Monash University, Melbourne, VIC 3004, Australia. kamila.kochan@monash.edu.

|February 6, 2026

Resumen

El diagnóstico rápido de la resistencia a los antimicrobianos (RAM) en enterococos es crucial. Un nuevo método utiliza espectroscopía y modelado para distinguir las cepas resistentes a la vancomicina (VRE) de las susceptibles a la vancomicina (VSE) en menos de dos horas.

Área de la Ciencia:

Microbiología
Espectroscopía
Química Computacional

Sus antecedentes:

La resistencia a los antimicrobianos (RAM) representa una amenaza significativa para la salud pública.
Los diagnósticos rápidos son esenciales para el tratamiento eficaz de las infecciones bacterianas.
La diferenciación entre enterococos resistentes a la vancomicina (VRE) y enterococos susceptibles a la vancomicina (VSE) es clínicamente importante.

Objetivo del estudio:

Desarrollar un enfoque de diagnóstico rápido para evaluar la resistencia a los antimicrobianos en enterococos.
Diferenciar entre enterococos resistentes a la vancomicina y enterococos susceptibles a la vancomicina en menos de dos horas.
Establecer una base para una herramienta de diagnóstico que apoye la toma de decisiones clínicas oportuna.

Principales métodos:

Se utilizó espectroscopía de infrarrojo por transformada de Fourier con reflectancia total atenuada (ATR-FTIR).
Se aplicó quimiometría y modelado basado en quimiometría para el análisis de datos.
Se identificaron marcadores espectrales tempranos que indican la acción de la vancomicina.

Principales resultados:

Se distinguió con éxito entre VRE y VSE en menos de dos horas.
Se predijeron con precisión los perfiles de resistencia bacteriana utilizando marcadores espectrales.
Se validó el método en muestras de Enterococcus conocidas y ciegas.

Conclusiones:

El enfoque desarrollado proporciona una base para una herramienta de diagnóstico rápido de RAM.
La determinación más rápida de la susceptibilidad bacteriana puede conducir a mejores resultados para los pacientes.
Este método apoya la toma de decisiones clínicas oportunas en el manejo de infecciones por enterococos.

Palabras clave:

Espectroscopía Enterococos Resistencia a los antimicrobianos Vancomicina Diagnóstico rápido Quimiometría Espectroscopía infrarroja por transformada de Fourier con reflectancia total atenuada VRE VSE

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