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Biosensor de Apta-Separada Anclada para Monitorización Continua de Vancomicina Basada en Fluorescencia Potenciada por
Gizem Aktug1,2, Naoto Asai3, Anil Bozdogan4
1FZU-Institute of Physics, Czech Academy of Sciences, Na Slovance 2, Prague 182 21, Czech Republic.
ACS sensors
|February 6, 2026
Resumen
Este estudio presenta un nuevo ensayo de apta-separada para la monitorización continua de analitos. El sensor de fluorescencia potenciada por plasmones ofrece límites de detección sintonizables para aplicaciones como la monitorización de antibióticos.
Área de la Ciencia:
- Ingeniería Biomédica
- Química Analítica
- Diagnóstico Molecular
Sus antecedentes:
- La monitorización continua de analitos de bajo peso molecular es crucial para el diagnóstico.
- Los métodos existentes pueden carecer de sensibilidad o capacidades en tiempo real.
- Los ensayos de apta-separada ofrecen una plataforma versátil para el reconocimiento molecular.
Objetivo del estudio:
- Desarrollar un ensayo de apta-separada con fluorescencia potenciada por plasmones para la monitorización continua de analitos.
- Demostrar la sintonizabilidad de las características del ensayo controlando las concentraciones superficiales.
- Validar el rendimiento del sensor para la detección del antibiótico vancomicina.
Principales métodos:
- Se utilizó una biointerfaz con segmentos de apta-separada (S1 y S2) en una superficie de sensor metálico.
- Se empleó un enlazador polimérico flexible (FPL) a base de ADN para anclar S1 y un fluoróforo.
- Se modularon los cambios de distancia inducidos por el analito entre la superficie y el fluoróforo para alterar la intensidad de la fluorescencia a través de efectos plasmónicos.
Principales resultados:
- El ensayo demostró interacciones de afinidad sintonizables, variando la constante de disociación para la vancomicina en dos órdenes de magnitud (nM a μM).
- Se logró un límite de detección sintonizable en el rango bajo de nM, adecuado para la monitorización continua.
- Se demostró la capacidad de modular las características clave del ensayo controlando las concentraciones superficiales.
Conclusiones:
- El ensayo de apta-separada desarrollado proporciona una plataforma sensible y adaptable para la monitorización continua de analitos.
- La lectura de fluorescencia potenciada por plasmones permite un control preciso del rendimiento del ensayo.
- Este enfoque es fácilmente adaptable a diversos analitos que utilizan interacciones de afinidad reversibles con apta-separadas confinadas en la superficie.
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