Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.7K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.7K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.8K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.8K
tRNA Activation02:26

tRNA Activation

23.0K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
23.0K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.5K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.5K
Activation Energy01:26

Activation Energy

86.8K
Activation energy is the minimum amount of energy necessary for a chemical reaction to move forward. The higher the activation energy, the slower the rate of the reaction. However, adding heat to the reaction will increase the rate, since it causes molecules to move faster and increase the likelihood that molecules will collide. The collision and breaking of bonds represents the uphill phase of a reaction and generates the transition state. The transition state is an unstable high-energy state...
86.8K
Secondary Active Transport01:55

Secondary Active Transport

138.1K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
138.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

PDS5A and TOP2B cooperate for chromatin recruitment via CTCF.

bioRxiv : the preprint server for biology·2026
Same author

Identification of novel regulators of LINE-1 expression via CRISPR/Cas9 screening.

Mobile DNA·2025
Same author

CTCF-RNA interactions orchestrate cell-specific chromatin loop organization.

Science advances·2025
Same author

Transcriptional interferences ensure one olfactory receptor per ant neuron.

Nature·2025
Same author

EPOP and MTF2 Activate PRC2 Activity through DNA-sequence specificity.

bioRxiv : the preprint server for biology·2025
Same author

A multiomic analysis of Waldenström macroglobulinemia defines distinct disease subtypes.

Blood·2025
Same journal

In This Issue.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Dolphins use names to remember whom to avoid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Small but mighty: The outsized role of small water bodies in the global carbon cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Functional traits produce conditional outcomes in different community contexts.

Proceedings of the National Academy of Sciences of the United States of America·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Feb 8, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

8.5K

EPOP y MTF2 activan la actividad de PRC2 a través de la especificidad de la secuencia de ADN

Jeffrey Granat1,2, Sanxiong Liu1,2,3,4,5, Luis Popoca1,2

  • 1HHMI, New York University Langone Health, New York, NY 10016.

Proceedings of the National Academy of Sciences of the United States of America
|February 6, 2026
PubMed
Resumen
Este resumen es generado por máquina.

El estudio revela que la proteína EPOP mejora la actividad de la metiltransferasa de histonas del Complejo Represor de Polycomb 2 (PRC2), crucial para la regulación génica. EPOP, junto con MTF2 y JARID2, promueve la deposición de H3K27me3 en secuencias de ADN específicas.

Palabras clave:
PRC2epigenéticacromatina represivatranscripción

Más Videos Relacionados

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.2K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

998

Videos de Experimentos Relacionados

Last Updated: Feb 8, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

8.5K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.2K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

998

Área de la Ciencia:

  • Epigenética
  • Biología Molecular
  • Biología de la Cromatina

Sus antecedentes:

  • El complejo represor de Polycomb 2 (PRC2) es esencial para la formación de heterocromatina facultativa y la expresión génica específica del tejido.
  • PRC2 cataliza la trimetilación de la lisina 27 de la histona H3 (H3K27me3), una marca dirigida por PRC1 para la compactación de la cromatina.
  • Cofactores como MTF2 y JARID2 guían a PRC2 a motivos de ADN específicos, regulando su actividad.

Objetivo del estudio:

  • Investigar el papel de EPOP en la regulación de la actividad de PRC2 y la deposición de H3K27me3.
  • Determinar si EPOP influye en el reclutamiento de PRC2 a la cromatina o en la actividad catalítica.
  • Elucidar los mecanismos cooperativos de EPOP, MTF2 y JARID2 en la función de PRC2.

Principales métodos:

  • Ensayos de metiltransferasa de histonas (HMT) in vitro para medir la actividad de PRC2.
  • Sistema de rescate de EED in vivo para evaluar el reclutamiento de PRC2 a la cromatina.
  • Ensayos de unión a dinucleosomas para evaluar la preferencia de secuencia de ADN de PRC2.

Principales resultados:

  • EPOP y MTF2 estimulan la actividad HMT de PRC2 in vitro.
  • EPOP no media el reclutamiento de PRC2 a la cromatina in vivo, pero coopera con MTF2 y JARID2 para la deposición de novo de H3K27me3.
  • EPOP mejora la unión de PRC2 a la cromatina de manera dependiente de la secuencia de ADN (secuencias ricas en GCN).

Conclusiones:

  • EPOP actúa como un regulador positivo de la actividad catalítica de PRC2, distinto de su papel en el reclutamiento a la cromatina.
  • EPOP, MTF2 y JARID2 colaboran para promover la deposición de H3K27me3 en secuencias de ADN específicas, contribuyendo a la formación de dominios de H3K27me3-cromatina.