Video Experimental Relacionado
Updated: Feb 8, 2026
10:24
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
Published on: December 3, 2017
10.9K
El factor de transcripción inducible por hipoxia regula el desarrollo de interneuronas embrionarias, la expresión de
I-Ling Lu1, Mengyi Song2, Cinthia Rangel-Sandoval3
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; Department of Pediatrics, Division of Neonatology, UCSF, San Francisco, CA 94158, USA; Department of Neurology, UCSF, San Francisco, CA 94143, USA.
Developmental cell
|February 6, 2026
Resumen
Los factores inducibles por hipoxia (HIF) son cruciales para el desarrollo cerebral, regulando la producción de neuronas inhibitorias y la función sináptica. Este estudio revela la señalización de HIF
Área de la Ciencia:
- Neurociencia; Biología Molecular; Biología del Desarrollo
Sus antecedentes:
- Los factores inducibles por hipoxia (HIF) regulan las respuestas celulares al oxígeno y al metabolismo en el cerebro.; El papel de los HIF en el desarrollo de neuronas inhibitorias no se comprende bien.
Objetivo del estudio:
- Investigar el papel de la señalización de HIF en el desarrollo de neuronas inhibitorias en la eminencia gangliónica medial (MGE).; Identificar los objetivos de HIF implicados en la proliferación de precursores de interneuronas y la sinaptogénesis.
Principales métodos:
- Eliminación condicional de Hif1/2a y von Hippel-Lindau (vHL) en la MGE de ratones.; Transcriptómica de célula única para identificar objetivos de HIF.; Análisis de interneuronas de parvalbumina (PV), sinapsis GABAérgicas y expresión de GRIN2B en el córtex de ratones adultos con deleción condicional de HIF1 (cKO).; Registros electrofisiológicos para evaluar la potenciación a largo plazo (LTP).
Principales resultados:
- La pérdida de Hif1/2a en precursores de la MGE redujo la proliferación, mientras que la pérdida de vHL la aumentó.; HIF1A se identificó como un activador directo de GRIN2B, que codifica la subunidad 2B del receptor NMDA.; Los ratones adultos con deleción condicional de HIF1 mostraron una reducción de interneuronas PV, menos sinapsis GABAérgicas y alteración de la LTP.
Conclusiones:
- La señalización de HIF desempeña un papel no canónico en la regulación de la producción de neuronas inhibitorias.; Los HIF son esenciales para la expresión de GRIN2B y la maduración de los circuitos corticales.; Esto resalta la importancia de los HIF en la función sináptica y el desarrollo de circuitos cerebrales.
Videos de Conceptos Relacionados
02:24Regulation of Expression Occurs at Multiple Steps
26.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.5K
02:24Regulation of Expression Occurs at Multiple Steps
4.0K
4.0K
02:23Master Transcription Regulators
7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
02:16Transcription Factors
82.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.8K
02:14GTPases and their Regulation
9.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
9.9K
01:23Regulation of Expression at Multiple Steps
1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K