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CLISGen: Un recurso completo de genotipos de SNP para líneas celulares humanas
Matteo Marchesin1, Davide Dalfovo2, Alessandro Romanel2
1Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Trento, Italy; University of Padova, Padova, Italy.
Journal of molecular biology
|February 8, 2026
Resumen
CLISGen simplifica la selección de líneas celulares de cáncer con genotipos específicos de polimorfismos de un solo nucleótido (SNP). Esta base de datos ayuda en la investigación en genómica funcional al proporcionar un fácil acceso a datos de SNP para más de 1000 líneas celulares de cáncer.
Área de la Ciencia:
- Genómica
- Investigación del cáncer
- Bioinformática
Sus antecedentes:
- La selección de líneas celulares con genotipos específicos de polimorfismos de un solo nucleótido (SNP) es un desafío importante en la genómica funcional.
- Este proceso a menudo requiere experiencia avanzada en bioinformática, lo que obstaculiza el progreso de la investigación.
Objetivo del estudio:
- Desarrollar CLISGen (Cell Lines SNP Genotypes), una base de datos y aplicación web fácil de usar.
- Simplificar y acelerar la selección de líneas celulares de cáncer basándose en genotipos de SNP.
Principales métodos:
- Se integraron y armonizaron datos de genotipos de SNP de secuenciación de genoma completo, exoma completo y ARN para más de 1000 líneas celulares de cáncer.
- Se enriqueció el conjunto de datos con información sobre alteraciones del número de copias y ascendencia genética.
- Se desarrolló una aplicación web para buscar variantes, genes o regiones genómicas con filtrado por tipo de tejido y calidad de los datos.
Principales resultados:
- CLISGen proporciona resultados gráficos y tabulares intuitivos para datos de genotipos de SNP.
- La plataforma facilita la búsqueda y filtrado eficientes de información genética de líneas celulares de cáncer.
- Se creó con éxito un recurso completo para más de 1000 líneas celulares de cáncer.
Conclusiones:
- CLISGen aborda eficazmente el cuello de botella en la selección de modelos celulares para la genómica funcional.
- La base de datos permite a los investigadores identificar de manera eficiente líneas celulares para estudiar las relaciones genotipo-fenotipo en el cáncer.
- CLISGen es un recurso valioso y de libre acceso para la comunidad de investigación del cáncer.
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