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El flujo lipofágico activado por PLIN3 libera FFAs para facilitar la propagación del CSFV

Bingke Li^1,2, Linke Zou^1,2, Chenchen Sun^1,2

¹College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

|February 9, 2026

Resumen

El virus de la fiebre porcina clásica (CSFV) secuestra el metabolismo de los lípidos del huésped al inducir la lipofagia, un proceso que degrada las gotas de lípidos. Este mecanismo, que involucra a la perilipina 3 (PLIN3) y la vía AMPK, apoya la replicación viral.

Área de la Ciencia:

Virología Virología.
Biología celular Biología celular.
La bioquímica metabólica es una bioquímica metabólica.

Sus antecedentes:

La infección por el virus de la fiebre porcina clásica (CSFV) altera el metabolismo de los lípidos del huésped.
Los mecanismos precisos que vinculan el CSFV y el metabolismo de los lípidos, particularmente la lipofagia, no se comprenden completamente.
La autofagia es inducida por el CSFV, pero su relación con la regulación de las gotas de lípidos no está clara.

Objetivo del estudio:

Para aclarar los mecanismos reguladores entre la infección por CSFV y la lipofagia del huésped.
Para investigar el papel de la perilipina 3 (PLIN3) en la lipofagia inducida por el VCSF y la replicación viral.
Para explorar la participación de la vía de señalización AMPK en este proceso.

Principales métodos:

Observación de la dinámica de las gotas de lípidos y la actividad de la autofagia durante la infección por el VCSF en las células esteladas hepáticas (HSC).
Investigación de la expresión de PLIN3 y su papel funcional utilizando técnicas de knockdown (shPLIN3).
Análisis de la activación de la vía de señalización de AMPK y la liberación de ácidos grasos libres (FFA).

Principales resultados:

La infección por CSFV aumentó inicialmente y luego disminuyó los niveles de gotas de lípidos, correlacionándose negativamente con la actividad de la autofagia.
El CSFV regula la expresión de PLIN3 en HSCs, lo que promueve la replicación viral.
PLIN3 activa la vía AMPK, mejorando la liberación de FFA mediada por la lipofagia, crucial para la replicación del CSFV.

Conclusiones:

CSFV induce la lipofagia a través de PLIN3 y la vía AMPK para aumentar las FFAs, apoyando la replicación viral.
PLIN3 es un mediador clave en la interacción entre el CSFV y el metabolismo de los lípidos del huésped.
Este estudio revela un nuevo mecanismo de interacción metabólica entre el virus y el huésped crucial para la propagación del CSFV.

Palabras clave:

AMPK es el nombre de AMPK.El CSFVV es el CSFVV.Las gotas de lípidos son gotas de lípidos.La lipofagia es la lipofagia.PLIN3 ((perilipina 3) es el nombre de la planta.

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