Video Experimental Relacionado

Updated: Feb 10, 2026

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

La citometría de masa unicelular revela firmas inmunes distintas en el cáncer colorrectal

Yu Yang¹, Botao Wang², Jing Xun³

¹School of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin, China.

Analytical methods : advancing methods and applications

|February 9, 2026

Resumen

La progresión del cáncer colorrectal (CRC) implica cambios significativos en las células inmunes. La citometría de masa reveló firmas inmunes distintas en pacientes con CCR, ofreciendo potencial para la detección temprana a través del análisis de sangre periférica.

Área de la Ciencia:

Inmunología Inmunología.
Oncología Oncología.
Biotecnología La biotecnología es la biotecnología.

Sus antecedentes:

El cáncer colorrectal (CRC) es una de las principales causas de muerte por cáncer a nivel mundial.
La inflamación está relacionada con el desarrollo del cáncer, pero las alteraciones de las células inmunes en el CRC no se comprenden completamente.
El análisis de sangre periférica ofrece un enfoque mínimamente invasivo para estudiar los cambios inmunológicos sistémicos en el CCR.

Objetivo del estudio:

Para caracterizar los cambios inmunofenotípicos durante la progresión del cáncer colorrectal (CRC) utilizando sangre periférica.
Para identificar firmas inmunes específicas de la enfermedad en donantes sanos, pacientes con adenoma y pacientes con CCR.
Evaluar el potencial de la citometría de masa (CyTOF) para el diagnóstico de CCR.

Principales métodos:

Análisis de 31 muestras no tratadas (donantes sanos, pacientes con adenoma, pacientes con CCR).
Utilizó citometría de masa de alto parámetro (CyTOF) para cuantificar >50 parámetros celulares simultáneamente.
Perfiles inmunofenotipicos comparativos en diferentes grupos de enfermedades.

Principales resultados:

Desregulación progresiva de los granulocitos y diferenciación alterada de las células B observada en el CRC.
Cambios específicos de las células T en el CRC: disminución de las células T de memoria central CCR4 + / CXCR3 +, aumento de las células T doblemente negativas CCR4 + y agotamiento de las células T CD8 +.
La heterogeneidad funcional de las células Natural Killer (NK) aumentó de donantes / adenomas sanos a CRC.

Conclusiones:

Las distintas firmas de células inmunes diferencian a los individuos sanos, los pacientes con adenoma y los pacientes con CCR.
El análisis de CyTOF revela cambios inmunofenotípicos significativos asociados con la progresión de la CCR.
Estos hallazgos respaldan el potencial de CyTOF como plataforma de diagnóstico para la detección y monitoreo tempranos de CCR.

Más Videos Relacionados

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Published on: May 27, 2020

Videos de Conceptos Relacionados

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...

Cancers Originate from Somatic Mutations in a Single Cell

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...

What is the Immune System?

What is the Immune System?

Atomic Mass

Atomic Mass

Atoms — and the protons, neutrons, and electrons that compose them — are extremely small. For example, a carbon atom weighs less than 2 × 10−23 g. When describing the properties of tiny objects such as atoms, we use appropriately small units of measure, such as the atomic mass unit (amu). The amu was originally defined based on hydrogen, the lightest element, then later in terms of oxygen. Since 1961, it has been defined with regard to the most abundant isotope of carbon, atoms of which...

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Cells of the Adaptive Immune Response

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...