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Identificación de un péptido de krill antártico (Euphausia superba) con actividad inmunorreguladora
Ruoting Hou1,2, Zhipeng Zheng1,2, Jinge Shao1,2
1Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. jiamiaohu@fafu.edu.cn.
Food & function
|February 10, 2026
Resumen
Los péptidos del krill antártico muestran potentes efectos estimulantes de la inmunidad. Un péptido específico, RDWPEGRG, mejora la función de los macrófagos y restaura la inmunidad en el pez cebra, lo que indica su potencial para los alimentos y nutracéuticos que mejoran la inmunidad.
Área de la Ciencia:
- La biotecnología marina es la biotecnología marina.
- Inmunología Inmunología.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El krill antártico (Euphausia superba) es una fuente de péptidos bioactivos.
- Los péptidos bioactivos pueden poseer propiedades inmunomoduladoras.
- La evaluación de los hidrolizados de krill para el refuerzo inmunológico es de interés.
Objetivo del estudio:
- Para investigar los efectos inmunorreguladores de los hidrolizados enzimáticos del krill antártico.
- Para aislar y caracterizar los péptidos bioactivos responsables de la mejora del sistema inmunológico.
- Para dilucidar los mecanismos de acción y validar la eficacia in vivo.
Principales métodos:
- Hidrólisis enzimática del krill antártico.
- Fraccionamiento mediante ultrafiltración y cromatografía.
- En ensayos in vitro con macrófagos RAW264.7 (proliferación, fagocitosis y secreción de NO).
- HPLC-MS/MS para la identificación de péptidos.
- Análisis de la expresión génica (IL-6, CCL2, CCL5).
- Pruebas de respiración mitocondrial.
- Estudio in vivo utilizando un modelo de inmunosupresión de pez cebra.
Principales resultados:
- El hidrolizado neutro de proteasa mostró la mayor actividad, promoviendo la proliferación de macrófagos.
- El péptido aislado RDWPEGRG mejoró significativamente la proliferación de macrófagos, la fagocitosis y la secreción de óxido nítrico.
- RDWPEGRG upregulated genes relacionados con el sistema inmunológico (IL-6, CCL2, CCL5) y el aumento de la tasa de consumo de oxígeno.
- RDWPEGRG restauró la competencia inmune en peces cebra inmunosuprimidos al mejorar la actividad de los macrófagos y normalizar el recuento de neutrófilos.
Conclusiones:
- RDWPEGRG de los hidrolizados del krill antártico es un potente péptido inmunostimulador.
- RDWPEGRG demuestra efectos inmunomoduladores significativos in vitro y in vivo.
- Este péptido tiene potencial como ingrediente funcional para alimentos que mejoran la inmunidad y aplicaciones nutracéuticas.
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