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Excavación de péptidos derivados del tallo de Lentinula edodes que inhiben doblemente la DPP-IV y la actividad de la
Qiulan Zheng1, Qian Kang1, Haosu Zhang1
1School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Bioscience, biotechnology, and biochemistry
|February 10, 2026
Resumen
Los nuevos péptidos de los tallos de hongos Lentinula edodes muestran doble inhibición de la dipeptidilpeptidasa-4 (DPP-IV) y de la enzima de conversión de la angiotensina I (ACE). Estos compuestos derivados de forma segura y natural ofrecen potencial para el manejo de la diabetes tipo 2 y la hipertensión.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Ciencias de la alimentación Ciencias de la alimentación.
- Farmacología Farmacología.
Sus antecedentes:
- La diabetes tipo 2 y la hipertensión frecuentemente coexisten, lo que requiere tratamientos dirigidos a ambas condiciones.
- La inhibición de la dipeptidil peptidasa-4 (DPP-IV) y la enzima de conversión de la angiotensina I (ACE) son estrategias terapéuticas clave.
- La proteína del tallo de Lentinula edodes es una fuente potencial de péptidos bioactivos debido a su perfil de aminoácidos.
Objetivo del estudio:
- Para derivar y caracterizar los péptidos de Lentinula edodes proteína madre con doble DPP-IV y actividad inhibidora de la ECA.
- Evaluar la eficacia y seguridad de estos nuevos péptidos.
Principales métodos:
- Hidrólisis enzimática de la proteína del tallo de Lentinula edodes utilizando la proteasa de Bacillus subtilis.
- Fraccionamiento e identificación de los péptidos activos.
- Ensayos in vitro para el DPP-IV y la inhibición de la ECA.
- Evaluación de la citotoxicidad utilizando células Caco-2.
- Síntesis en fase sólida de péptidos identificados.
Principales resultados:
- Una fracción específica (F1-2) demostró una potente inhibición tanto de la DPP-IV (IC50 = 0.0910 mg/mL) como de la ECA (IC50 = 0.0189 mg/mL).
- Se sintetizaron tres nuevos péptidos (EW-1, WR-2, LP-3), que muestran una inhibición >60% de ambas enzimas a 1 mg/mL.
- Los péptidos sintetizados no mostraron toxicidad significativa para las células Caco-2.
Conclusiones:
- Los péptidos derivados de los tallos de Lentinula edodes poseen importantes propiedades inhibidoras de la DPP-IV y de la ECA.
- Estos péptidos no son tóxicos y representan candidatos prometedores para alimentos funcionales o productos farmacéuticos.
- Esta investigación apoya el desarrollo de productos de salud naturales para el manejo de la diabetes tipo 2 y la hipertensión.
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