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Cápsulas COF huecas que co-inmobilizan enzimas y nanoenzimas: un nanorreactor en cascada biomimético para el

Zhifei Cheng¹, Baozhu Zhao¹, Jin Mao²

¹Engineering Research Center of Bio-Process, Ministry of Education, Key Laboratory for Agricultural Products Modern Processing of Anhui Province, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P. R. China.

Analytical chemistry

|February 10, 2026

Resumen

Desarrollamos cápsulas de marco orgánico covalente hueco (COF) para la inmovilización de enzimas. Estas nuevas cápsulas mejoran las reacciones en cascada y las aplicaciones de biosensores, superando las limitaciones anteriores en la estabilidad y actividad de las enzimas.

Área de la Ciencia:

Ciencia de los materiales Ciencia de los materiales.
Biotecnología La biotecnología es la biotecnología.
Ingeniería Química Ingeniería Química.

Sus antecedentes:

Las reacciones en cascada que combinan biocatálisis y catálisis química son prometedoras, pero se ven obstaculizadas por la inestabilidad de las enzimas y los problemas de transferencia de masa.
Los métodos de inmovilización enzimática existentes a menudo se enfrentan a desafíos con la compatibilidad de la enzima portadora y la preservación de la actividad enzimática.

Objetivo del estudio:

Desarrollar una nueva estrategia para fabricar cápsulas de marco orgánico covalente hueco (COF) para una inmovilización enzimática eficiente.
Para crear una plataforma versátil para biosensing avanzado y reacciones en cascada.

Principales métodos:

Una estrategia de crecimiento epitaxial heterogéneo asistido por polímeros utilizando ZIF-8 como plantilla de sacrificio.
La doble modificación de ZIF-8 con polivinilpirrolidona (PVP) y polietilenimina ramificada (BPEI) para controlar el crecimiento de la FOC.
Fabricación de cápsulas huecas de COF (HNKCOF-98) con una gran superficie (1623 m2/g).

Principales resultados:

Las cápsulas HNKCOF-98 se sintetizaron con éxito con alta cristalinidad y área de superficie.
Las enzimas inmovilizadas dentro de HNKCOF-98 mantuvieron flexibilidad conformacional y alta actividad.
El sistema GOX&HRP@HNKCOF-98 demostró un excelente rendimiento en la detección de glucosa.
El reactor biomimético XOD@HNKCOF-98@Pt mostró detección sensible de xantina.

Conclusiones:

La estrategia asistida por polímeros desarrollada proporciona una plataforma versátil para crear diversas morfologías de COF.
Las cápsulas huecas de COF ofrecen una solución efectiva para la inmovilización de enzimas, mejorando la estabilidad y la actividad.
Este enfoque permite sistemas de cascada de enzimas de alto rendimiento y biosensores sensibles.

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