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Biblioteca de referencia de ADN 12S casi completa para los peces de agua dulce de la Guayana Francesa, región del
Sébastien Brosse1, Yves Cuenot2, Céline Condachou2
1Centre de Recherche sur la Biodiversité et l'Environnement, CRBE UMR5300 UT-CNRS-IRD-INP, Université Toulouse, 118 route de Narbonne, CEDEX 4, 31062, Toulouse, France. sebastien.brosse@utoulouse.fr.
Scientific data
|February 10, 2026
Resumen
Una nueva biblioteca de referencia de ADN para la Guayana Francesa.
Área de la Ciencia:
- Ecología Molecular Ecología Molecular
- Biodiversidad Ciencia Ciencia de la biodiversidad.
- Ictiología Ictiología.
Sus antecedentes:
- Las técnicas de ADN ambiental (eDNA) son cruciales para los inventarios de biodiversidad.
- Las bibliotecas de referencia de ADN limitadas obstaculizan las aplicaciones de ADN electrónico, especialmente en regiones pobres en datos.
- La diversidad de peces de agua dulce de la Guayana Francesa es rica, pero subasamplada.
Objetivo del estudio:
- Para crear una biblioteca de referencia completa de ADN mitocondrial 12S para los peces de agua dulce de la Guayana Francesa.
- Para apoyar las evaluaciones de la biodiversidad utilizando el metabarcoding de eDNA.
- Para llenar un vacío crítico de datos de biodiversidad en los Neotrópicos.
Principales métodos:
- Extracción de ADN de 1.439 especímenes recolectados en el campo y 118 especímenes de museos.
- Secuenciación del gen mitocondrial 12S.
- Dirigido a dos primers de ADN electrónico de peces comunes (Teleo1 y 12S-V5).
Principales resultados:
- La biblioteca incluye 1.557 referencias que cubren 369 especies (88,92% de los peces de agua dulce de la Guayana Francesa).
- Abarca la diversidad intraspecífica para la mayoría de las especies.
- El conjunto de datos representa el 6,31% de las especies de peces neotrópicos, el 23,05% de los géneros y el 56,04% de las familias.
Conclusiones:
- Este conjunto de datos establece una robusta biblioteca de referencia de ADN para peces de agua dulce neotropical.
- Mejora significativamente la capacidad de identificación de peces basada en ADN electrónico en la región.
- La biblioteca es valiosa para el monitoreo y la investigación de la biodiversidad regional.
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