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Efectos combinatorios comparativos de las endolisinas LNT103 con diez antibióticos convencionales contra patógenos

Jaehak Jo¹, Heejoon Myung^1,2,3

¹Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yong-In, Gyeonggi-do 17035, Republic of Korea.

Journal of microbiology and biotechnology

|February 11, 2026

Resumen

Las endolisinas de los bacteriófagos muestran efectos sinérgicos con los antibióticos contra las bacterias gramnegativas, particularmente cuando las bacterias son susceptibles a la endolisina. Esto guía las terapias de combinación optimizadas.

Área de la Ciencia:

Microbiología Microbiología.
Farmacología Farmacología.
Enfermedades infecciosas Enfermedades infecciosas.

Sus antecedentes:

Las endolisinas derivadas de bacteriófagos son agentes antibacterianos potenciales.
Sus combinaciones con antibióticos contra las bacterias gramnegativas necesitan más estudios.

Objetivo del estudio:

Evaluar las interacciones entre la endolisina LNT103 y los antibióticos en bacterias gramnegativas.
Para identificar los factores que influyen en la eficacia de la combinación.

Principales métodos:

Los ensayos de tablero de ajedrez determinaron MICs y FICIs para 300 combinaciones.
Se probó endolysin LNT103 con 10 antibióticos contra E. coli, P. aeruginosa y A. baumannii.
Los ensayos de tiempo muerto evaluaron la actividad bactericida.

Principales resultados:

Las interacciones sinérgicas fueron dependientes de la cepa, observadas con cloranfenicol, sulfametoxazol, colistina y trimetoprima.
Los efectos positivos de la combinación fueron más frecuentes en P. aeruginosa (76,0%), correlacionados con MICs de endolisina más bajos.
La concentración de endolisina influyó en la actividad bactericida más que la concentración de antibióticos.

Conclusiones:

La susceptibilidad intrínseca a la endolisina es clave para la eficacia de la combinación.
Los resultados ofrecen un marco para optimizar las terapias con antibióticos de endolisina contra patógenos gramnegativos multirresistentes.

Palabras clave:

La resistencia a los antibióticos es la resistencia a los antibióticos.La endolisina de los bacteriófagos Tratamiento de combinación.La sinergia es la sinergia.

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