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Aplicación intravaginal de progesterona como una herramienta eficiente y reproducible para sincronizar el ciclo estro

Ban Sato¹, Yutaka Yamashita¹, Miyuki Shindo²

¹Laboratory of Regulatory Biology, Department of Life Sciences School of Agriculture, Meiji University Kawasaki Kanagawa Japan.

Reproductive medicine and biology

|February 11, 2026

Resumen

La crema intravaginal de progesterona (P4) sincroniza efectivamente el ciclo estro del ratón. Este método mejora la eficiencia reproductiva y el bienestar animal al reducir la incomodidad y la necesidad de varones vasectomizados.

Área de la Ciencia:

Biología de la reproducción Biología reproductiva.
Los modelos animales son modelos de animales.
Las ciencias veterinarias son la ciencia veterinaria.

Sus antecedentes:

La sincronización del ciclo de estro es vital para la consistencia de la investigación reproductiva en ratones hembras.
La administración convencional de progesterona tiene limitaciones como la invasividad y la eficacia inconsistente.

Objetivo del estudio:

Desarrollar y evaluar la aplicación intravaginal de progesterona (P4) para la sincronización del ciclo estro en ratones.
Evaluar el impacto de este nuevo método en el rendimiento reproductivo y el éxito de la transferencia de embriones.

Principales métodos:

La crema personalizada de progesterona (P4) se aplica por vía intravaginal y se retira después de 24 horas.
Pruebas de frotis vaginales utilizadas para el monitoreo del ciclo estro durante 4-5 días.
Combinación de P4 con anticuerpo anti-inhibina (AIMA) para mejorar los resultados reproductivos y la transferencia de embriones.

Principales resultados:

La aplicación intravaginal de P4 sincronizó con éxito los ciclos estrosos sin efectos negativos en las tasas de embarazo o el tamaño de la camada.
El tratamiento combinado de P4 y AIMA aumentó significativamente el número de crías en comparación con el apareamiento natural (p = 0,006).
El protocolo facilitó la producción consistente de receptores pseudopregnantes para la transferencia de embriones.

Conclusiones:

La P4 intravaginal ofrece un método menos invasivo y más eficaz para la sincronización del ciclo estro del ratón.
Este enfoque mejora la eficiencia reproductiva y el bienestar animal en los entornos de investigación.
El método reduce la dependencia de los varones vasectomizados, simplificando los procedimientos experimentales.

Palabras clave:

Bienestar animal bienestar de los animales.aplicaciones intravaginales.La progesterona es la progesterona.Las hembras pseudopregnantes son hembras pseudopregnantes.sincronización del ciclo de estro.

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