Video Experimental Relacionado
Updated: Feb 12, 2026
09:01
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
14.8K
El knockdown de CRIP1 mejora la dependencia glicolítica y aumenta la sensibilidad a la 2-Deoxy-D-Glucose en la
Muhammad Asif Zeb1, Faryal Mehwish Awan1, Aamir Ali Khan2
1Department of Medical Lab Technology, The University of Haripur, Haripur, 22620, Khyber Pakhtunkhwa, Pakistan.
Molecular biology reports
|February 11, 2026
Resumen
El derribo de la proteína intestinal rica en cisteína 1 (CRIP1) en las células de la leucemia mieloide aguda (LMA) aumenta la glucólisis. Esto hace que las células de LMA sean más sensibles al inhibidor glicolítico 2-deoxy-D-glucosa (2-DG), lo que sugiere el 2-DG como una terapia potencial.
Área de la Ciencia:
- Las malignidades hematológicas.
- El metabolismo del cáncer.
- Oncología Molecular Oncología Molecular
Sus antecedentes:
- La leucemia mieloide aguda (LMA) es un cáncer agresivo con malos resultados de tratamiento.
- Las células cancerosas reprograman el metabolismo, a menudo confiando en la glucólisis.
- Se desconoce el papel de la proteína intestinal rica en cisteína 1 (CRIP1) en el metabolismo de la LMA.
Objetivo del estudio:
- Para investigar los efectos metabólicos de la reducción de CRIP1 en la LMA.
- Para evaluar la eficacia de 2-deoxy-D-glucose (2-DG) y IACS-010759 en las células de LMA de CRIP1-nockdown.
Principales métodos:
- La reducción de CRIP1 se estableció en las células OCI-AML3.
- Se midió el consumo de glucosa y la producción de lactato.
- La muerte celular se evaluó mediante citometría de flujo después del tratamiento con 2-DG o IACS-010759.
Principales resultados:
- El knockdown de CRIP1 aumentó significativamente la absorción de glucosa y la producción de lactato.
- Las células noqueadas de CRIP1 mostraron un aumento de la expresión de LDHA.
- El tratamiento con 2-DG condujo a una mayor muerte celular en las células CRIP1-nockdown en comparación con IACS-010759.
Conclusiones:
- El bloqueo de CRIP1 induce un cambio glicolítico en las células de LMA.
- Las células de LMA con CRIP1 knockdown son sensibles al 2-DG.
- El estado de CRIP1 puede predecir la respuesta a terapias metabólicas como el 2-DG.
Videos de Conceptos Relacionados
01:24Other Glycolytic Pathways
990
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
990
03:00Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
01:18Increasing Function
401
An increasing function exhibits a rise in output values as input values increase. This behavior is depicted graphically as a curve or line that slopes upward from left to right. Such a function satisfies the condition that if x1 < x2, then f(x1) < f(x2), indicating that the function values grow with increasing inputs. This concept is fundamental in understanding growth trends across various domains, such as population dynamics, financial investments, or resource consumption.The...
401
01:02Glucose Homeostasis: Regulation of Blood Glucose
4.4K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
4.4K
01:21Frequency-dependent Selection
24.2K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
24.2K
02:55Temperature Dependence on Reaction Rate
89.3K
The Collision Theory
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
89.3K