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Integrative GWAS y RNA-seq identifican a MYL9 como un regulador clave de la resistencia a la enfermedad pullorum en

Jiongwen Wu¹, Zhexia Fan¹, Xiaohuan Chao¹

¹Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510000, China; State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510000, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510000, China.

Poultry science

|February 11, 2026

Resumen

Este estudio identifica a MYL9 como un gen clave en la inmunidad de las aves contra la enfermedad de Pullorum. MYL9 aumenta significativamente la expresión del factor inmune, ofreciendo un objetivo potencial para la resistencia a las enfermedades.

Área de la Ciencia:

Inmunología aviar.
La genómica es la genómica.
Salud de las aves de corral.

Sus antecedentes:

La enfermedad de Pullorum, causada por Salmonella Pullorum, es una gran amenaza para las aves de corral.
Comprender las respuestas inmunes del huésped es crucial para el control de enfermedades.

Objetivo del estudio:

Para investigar la respuesta inmune del huésped y la regulación génica durante la infección por Salmonella Pullorum en gallinas de White Plymouth Rock.
Para identificar los factores genéticos asociados con la resistencia a las enfermedades.

Principales métodos:

Estudio de asociación de todo el genoma (GWAS) y secuenciación de ARN (RNA-seq) en 375 gallinas.
Análisis de los niveles de factores inmunes (IgG, IL-6, IL-8) y la expresión génica.
Validación funcional de genes candidatos en las células HD11.

Principales resultados:

GWAS identificó 72 SNPs y 289 genes candidatos.
La secuenciación de ARN reveló 536 genes de expresión diferencial (DEG) relacionados con la infección por Salmonella y las vías inmunológicas.
Se identificaron cinco genes superpuestos (MYL9, SYT1, KLHL38, C11orf87, KCNH4), con MYL9 en el centro de la vía de infección por Salmonella.
MYL9 aumentó significativamente la regulación de los factores inmunes (IL-2, IL-3, IL-6, IFN-α, IFN-β, TNF-α) y las proteínas (IFN-α, TNF-α, IgG) en los ensayos celulares.

Conclusiones:

MYL9 juega un papel crítico en la respuesta inmune de las aves de corral a Salmonella Pullorum al mejorar la producción de factores inmunes.
MYL9 representa un objetivo genético potencial para mejorar la resistencia de las aves de corral a la enfermedad de Pullorum.

Palabras clave:

Estudio de asociación de todo el genoma.HD11 HD11 HD11 HD11 HD11 HD11 HD11 HD11 HD11 HD11 MYL9 es para mí.El ARN-seqqq es el ARN.Salmonella Pullorum también está disponible.

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