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Eliminación, transformación y cambios de toxicidad de las halobenzoquinonas por las enzimas digestivas

Liu He¹, De-Xiu Wu¹, Wen-Min Wang¹

¹Shenzhen Key Laboratory of Ecological Remediation and Carbon Sequestration, Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Institute of Environment and Ecology, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China; Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Institute of Environment and Ecology, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China.

|February 11, 2026

Resumen

Las enzimas digestivas eliminan y transforman significativamente las halobenzoquinonas tóxicas (HBQ), reduciendo sus riesgos para la salud. La digestión intestinal es la más efectiva, pero permanece cierta toxicidad, lo que afecta a las evaluaciones de seguridad del agua potable.

Área de la Ciencia:

Química del medio ambiente Química del medio ambiente
Toxicología Toxicología.
La bioquímica es la bioquímica.

Sus antecedentes:

Las halobenzoquinonas (HBQ) son subproductos tóxicos de la desinfección resistentes al tratamiento convencional del agua.
Su destino y transformación durante la digestión humana son en gran parte desconocidos.
Comprender el comportamiento de la HBQ en el tracto gastrointestinal es crucial para la evaluación del riesgo para la salud.

Objetivo del estudio:

Para investigar la eliminación, transformación y cambios de toxicidad de HBQs mediados por enzimas digestivas.
Para dilucidar los mecanismos de las interacciones enzima-HBQ.
Para evaluar el impacto de la degradación enzimática en la toxicidad del HBQ.

Principales métodos:

Digestión simulada utilizando la amilasa salival, la pepsina y la tripsina.
Estudios de afinidad de unión enzimática y simulaciones QM/MM.
Análisis de los productos de transformación y evaluación de la citotoxicidad y genotoxicidad utilizando ensayos de organoides del colon.

Principales resultados:

La digestión simulada aceleró significativamente la eliminación de HBQ, con la digestión intestinal mostrando la mayor eficiencia (87.196.0%).
El 2,6-DCBQ mostró la mayor afinidad de unión a las enzimas digestivas.
La acción enzimática convirtió los enlaces C = O, formó adutos covalentes con enzimas y redujo la citotoxicidad y genotoxicidad de HBQ al generar menos productos de estrés oxidativo.

Conclusiones:

Las enzimas digestivas juegan un papel crítico en la eliminación y transformación de HBQ dentro del cuerpo humano.
La degradación enzimática reduce la toxicidad del HBQ, pero no la elimina por completo.
Estos hallazgos proporcionan información crucial para evaluar los riesgos para la salud humana asociados con los HBQ en el agua potable.

Palabras clave:

Los organoides del colon se encuentran en el colon.La desintoxicación es la desintoxicación.Las enzimas digestivas son enzimas digestivas.Las halobenzoquinonas son las halobenzoquinonas.La cinética de la eliminación Las vías de transformación.

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