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Disminución de la estabilidad biomecánica corneal e integridad estructural en ratones knockout PPAR-α
Chengfang Zhu1, Huan He2, Hongwei Yan3
1Eye Institute and Affiliated Xiamen Eye Center of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Affiliated Hospital of Putian University.
Experimental eye research
|February 11, 2026
Resumen
Los ratones que carecen de receptor activado por el proliferador peroxisomático-α (PPAR-α) muestran una reducción de la estabilidad biomecánica de la córnea y la integridad microestructural. Esta disminución está relacionada con componentes alterados de la córnea, la inflamación y la actividad de la proteasa.
Área de la Ciencia:
- Oftalmología Oftalmología.
- Ciencia de los Biomateriales Ciencia de los Biomateriales.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La insuficiencia biomecánica corneal está relacionada con las citoquinas inflamatorias y las enzimas proteolíticas.
- La activación del receptor activado por el proliferador peroxisomático-α (PPAR-α) puede inhibir las cascadas inflamatorias.
Objetivo del estudio:
- Investigar las características biomecánicas y estructurales de la córnea en ratones PPAR-α-/-.
- Evaluar las alteraciones en las citoquinas y enzimas inflamatorias en ratones PPAR-α-/-.
Principales métodos:
- Corneas evaluadas de PPAR-α-/- y ratones de tipo salvaje utilizando pruebas de elasticidad, digestión enzimática, OCT, IVCM, TEM y RT-PCR.
- Tiempo estimado de ruptura de la película lagrimal (tBUT) y tinción de sodio con fluoresceína corneal.
Principales resultados:
- Los ratones PPAR-α-/- mostraron una disminución del módulo tangente de la córnea, resistencia enzimática y córneas más delgadas.
- La microscopia reveló fibras estomales desorganizadas y densidad reducida en ratones PPAR-α-/-.
- El análisis de la expresión génica indicó una disminución de las proteínas estructurales y un aumento de los marcadores inflamatorios (TNF-α, MMP-9) en las córneas PPAR-α-/-.
Conclusiones:
- La deficiencia de PPAR-α compromete la estabilidad biomecánica de la córnea y la integridad microestructural.
- La desregulación de los componentes estructurales, los factores inflamatorios y las proteasas contribuye a las alteraciones de la córnea en los ratones PPAR-α-/-.
Palabras clave:
Biomecánica de la córnea biomecánica de la córnea.módulo de elasticidad del módulo de elasticidad.digestión del tejido enzimático digestión del tejido.microscopía confocal in vivo con el microscopio confocal.Peroxisoma proliferador activado por el receptor-α.Más Videos Relacionados
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