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Updated: Feb 13, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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Published on: August 20, 2021

Identificación de sitios de inserción de transgenes mediante secuenciación del genoma completo de lecturas cortas y

L Kaplan, S J Edgerton, B D Mahoney

bioRxiv : the preprint server for biology

|February 12, 2026

Abstract:

Transgenic mouse lines are essential to uncover organ or system level genotype-phenotype relationships. The generation of such lines via transgene addition may lead to the insertion into unknown genomic loci potentially leading not only to the disruption of native genes but also attenuation of transgene expression. Additionally, this often results in the inability to determine transgene zygosity which in turn complicates breeding and interpretation of experimental results. In this study we present two whole genome sequencing based pipelines that allow the identification and genotyping of even complex multi transgenic inserts. As they use widely available reagents and bioinformatic tools, they can easily be applied to develop genotyping strategies in potentially any species.

Palabras clave:

secuenciación del genoma completo transgénico genotipado ratón inserción de transgenes

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