Video Experimental Relacionado
Updated: Feb 14, 2026
08:32
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
Published on: March 10, 2017
10.6K
Una breve guía para aislar los hepatocitos primarios de ratones
Jisoo Song1, Garam An1, Gwonhwa Song2
1Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Molecules and cells
|February 12, 2026
Resumen
Los investigadores optimizaron un protocolo para aislar los hepatocitos primarios de ratones. Este método mejora el rendimiento y la viabilidad celular, ayudando a la fisiología del hígado y la investigación de enfermedades.
Área de la Ciencia:
- Ciencias hepáticas y biliares Ciencias hepáticas y biliares
- Biología celular Biología celular.
- Investigación biomédica en la investigación biomédica.
Sus antecedentes:
- Los hepatocitos primarios son esenciales para el estudio de la fisiología y las enfermedades del hígado.
- Los métodos de aislamiento actuales se enfrentan a desafíos para lograr una alta viabilidad celular debido a la complejidad técnica y la fragilidad celular.
Objetivo del estudio:
- Presentar un protocolo optimizado para el aislamiento de hepatocitos primarios de ratón.
- Para mejorar el rendimiento y la viabilidad de los hepatocitos aislados para la investigación.
Principales métodos:
- Desarrollo y perfeccionamiento de un protocolo de aislamiento de hepatocitos.
- Centrarse en las consideraciones clave para mejorar la supervivencia celular y la recuperación.
Principales resultados:
- Alcanzó un alto rendimiento de hepatocitos primarios.
- Se mantuvo la alta viabilidad de los hepatocitos aislados.
- Proporcionó un método confiable para la investigación del hígado.
Conclusiones:
- El protocolo optimizado facilita el aislamiento de hepatocitos de alta calidad.
- Esto contribuye al avance de la investigación biomédica relacionada con el hígado.
- Permite estudios más robustos en fisiología y patología hepática.
Videos de Conceptos Relacionados
01:47Primary Active Transport
201.0K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
201.0K
01:29Primary Active Transport
14.5K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
14.5K
03:02Primary and Secondary Growth in Roots and Shoots
60.7K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.7K
01:06Primary Production
25.5K
The total amount of energy acquired by primary producers in an ecosystem is called gross primary production (GPP). However, of this energy, producers use some for metabolic processes, and some is lost as heat, decreasing the amount of energy available to the next trophic level. The remaining usable amount of energy is called the net primary productivity (NPP). In terrestrial ecosystems, NPP is driven by climate, while light penetration and nutrient availability drive NPP in aquatic ecosystems.
25.5K
01:28Primary Distribution
556
Primary distribution systems deliver electrical power from substations to consumers through various voltage classes, with 15-kV class voltages being predominant among U.S. utilities. Older 2.5- and 5-kV classes are being replaced by 15-kV primaries, while higher 25- to 34.5-kV classes are used in high-density urban areas and rural regions with long feeders. Three-phase, four-wire multigrounded systems are widely employed for balanced power delivery, using the neutral wire as a grounding point.
556
01:17Nomenclature of Primary Amines
4.6K
Primary, secondary, and tertiary amines are compounds consisting of one, two, and three alkyl groups connected to the amino group (–NH2), respectively. As depicted in Figure 1, the common name of the primary amines is obtained by adding the suffix -amine to the alkyl substituent attached to the amino group as the corresponding alkylamine.
4.6K