Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

G-protein Coupled Receptors01:21

G-protein Coupled Receptors

132.2K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
132.2K
Protein and Protein Structure02:15

Protein and Protein Structure

89.4K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
89.4K
Structural Protein Function01:56

Structural Protein Function

30.1K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.1K
Structural Protein Function01:56

Structural Protein Function

3.3K
3.3K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

17.8K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
17.8K
Protein and Protein Structures02:15

Protein and Protein Structures

19.3K
19.3K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Cryo-electron microscopy structures of human cone visual pigments.

Science (New York, N.Y.)·2026
Same author

Ultrarapid MC1R protein and associated plumage color evolution in the domestic chicken.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A mononuclear nonheme iron complex with higher affinity for O<sub>2</sub> than CO via hydrogen bonding.

Nature communications·2026
Same author

The Conformational Switches of a Bacterial Light-Driven Sodium Pump Characterized by Time-Resolved Resonance Raman Spectroscopy.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

A Cryptic Pocket Allosterically Modulates Oligosaccharide Binding to DC-SIGN.

JACS Au·2026
Same author

Multicolor photoreactions of the red light-activated channelrhodopsin Chrimson.

Proceedings of the National Academy of Sciences of the United States of America·2025

Video Experimental Relacionado

Updated: Feb 15, 2026

Quantifying Agonist Activity at G Protein-coupled Receptors
11:45

Quantifying Agonist Activity at G Protein-coupled Receptors

Published on: December 26, 2011

19.8K

Cambios Estructurales de la Proteína y del Cromóforo Acoplados a Protones Controlan la Activación del Fitocromo

Galaan Merga1, Maximilian Große1, Anastasia Kraskov2

  • 1Humboldt- Universität Zu Berlin, Institut für Biologie, Biophysikalische Chemie, Invalidenstr 42, Berlin D-10115, Germany.

Biochemistry
|February 13, 2026
PubMed
Resumen

Los fitocromos utilizan la transferencia de protones para cambiar entre estados, desencadenando cambios estructurales. Esta transferencia de protones intramolecular es esencial para la función del fitocromo y las transiciones de estructura secundaria en general.

Palabras clave:
fitocromoespectroscopíatransferencia de protonescambios estructuralesbiología molecularbioquímica

Más Videos Relacionados

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.1K
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

16.3K

Videos de Experimentos Relacionados

Last Updated: Feb 15, 2026

Quantifying Agonist Activity at G Protein-coupled Receptors
11:45

Quantifying Agonist Activity at G Protein-coupled Receptors

Published on: December 26, 2011

19.8K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.1K
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

16.3K

Área de la Ciencia:

  • Bioquímica
  • Biología Molecular
  • Espectroscopía

Sus antecedentes:

  • Los fitocromos son sensores de luz que controlan procesos fisiológicos.
  • La fotoisomerización inicia la activación del fitocromo, involucrando estados intermedios como Meta-Rc.
  • El estado Meta-Rc es crucial para la formación de Pfr y la transición de la estructura de la lengua del fitocromo.

Objetivo del estudio:

  • Investigar la estructura y las reacciones del estado Meta-Rc en el fitocromo bacteriano Agp1.
  • Elucidar el papel de la migración de protones en la señalización del fitocromo.
  • Determinar el mecanismo de las transiciones de estructura secundaria en los fitocromos.

Principales métodos:

  • Espectroscopía de diferencias infrarrojas (IR).
  • Espectroscopía de resonancia Raman.
  • Se estudió el fitocromo Agp1 a diferentes temperaturas y pH.

Principales resultados:

  • La formación de Meta-Rc implica la enolización y desprotonación del cromóforo; la desintegración implica la reprotonación.
  • La migración de protones desencadena la transición de la estructura secundaria de la lengua (interconversión β-lámina/α-hélice).
  • Se observa un equilibrio conformacional dependiente del pH en los estados Meta-Rc y Pfr.

Conclusiones:

  • Las transiciones de estructura secundaria son inducidas por la transferencia de protones vinculada al cromóforo, no por la relajación del cromóforo.
  • La transferencia de protones intramolecular es un requisito previo para las transiciones de estructura secundaria en los fitocromos.
  • Los hallazgos respaldan un mecanismo general para la señalización del fitocromo en diferentes especies.