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Una sonda fluorescente de pH sensible con gran desplazamiento de Stokes y transición estrecha para la imagen de

Bochao Chen¹, Jixiang Zhang¹, Yifan Zhong¹

¹School of Pharmacy, Shandong Second Medical University, Weifang, 261053, China.

|February 13, 2026

Resumen

Una nueva sonda fluorescente de pH, NAM, ofrece una detección precisa de cambios sutiles en el pH celular. Esta herramienta representa con precisión las variaciones de pH en las fases del ciclo celular, lo que avanza nuestra comprensión de la biología celular y las enfermedades.

Área de la Ciencia:

Biología Celular
Bioquímica
Biología Química

Sus antecedentes:

El pH intracelular es fundamental para las funciones celulares y el desarrollo de enfermedades.
Las sondas de pH existentes tienen limitaciones como rangos dinámicos amplios, lo que provoca imágenes borrosas y resultados inexactos.
La detección precisa de cambios sutiles de pH es esencial para comprender los procesos celulares.

Objetivo del estudio:

Diseñar y sintetizar una novedosa sonda fluorescente de pH (NAM) con alta sensibilidad y un rango de transición estrecho.
Investigar el mecanismo de respuesta al pH en la nueva sonda.
Demostrar la utilidad de la sonda en la imagen de la dinámica del pH intracelular, incluso en las fases del ciclo celular.

Principales métodos:

Síntesis de una sonda fluorescente basada en anhídrido 1,8-naftálico (NAM).
Caracterización de la respuesta al pH de NAM, pKa y propiedades de fluorescencia.
Aplicación de NAM para la imagen del pH intracelular en lisosomas y en diferentes fases del ciclo celular.

Principales resultados:

NAM exhibe un rango de transición de pH estrecho (2,4 unidades) y una mejora de la fluorescencia de 15 veces.
La sonda tiene un pKa de 6,71 y un gran desplazamiento de Stokes (112 nm), lo que indica una transferencia de carga intramolecular eficiente.
NAM detectó con éxito fluctuaciones de pH inducidas por LPS, privación de nutrientes y cloroquina.
La novedosa imagen de pH a través de las fases del ciclo celular reveló alcalinización de la fase G0/G1 a la fase S y acidificación en la fase G2/M.

Conclusiones:

NAM es una sonda fluorescente altamente sensible y específica para la detección precisa del pH intracelular.
La sonda permite obtener nuevas perspectivas sobre los cambios dinámicos de pH durante el ciclo celular.
NAM es prometedor para investigar procesos fisiológicos y patológicos relacionados con el pH.

Palabras clave:

Ciclo celular Sonda fluorescente Transición estrecha pH

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