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Marcos Olivera-Gómez¹, Guadalupe Cumplido-Laso¹, Dixan Agustín Benitez¹

¹Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, Badajoz, 06071, Spain.

Scientific reports

|February 15, 2026

Resumen

La caveolina-1 (Cav-1) interviene en la reparación del epitelio de las vías respiratorias. Su ausencia acelera la diferenciación de las células multiciliadas (MCC), lo que sugiere que la Cav-1 modula este proceso a través de la señalización Notch.

Área de la Ciencia:

Biología respiratoria
Biología celular y molecular
Biología epitelial

Sus antecedentes:

El epitelio de las vías respiratorias es crucial para la salud respiratoria, defendiendo contra los insultos ambientales.
Las células madre basales (BSCs) impulsan la reparación epitelial a través de la autorrenovación y la diferenciación.
La caveolina-1 (Cav-1) se expresa en las células madre basales de las vías respiratorias y en las células multiciliadas (MCCs), pero su función en la diferenciación no está clara.

Objetivo del estudio:

Investigar el papel de la caveolina-1 (Cav-1) en la diferenciación de células madre basales (BSCs) in vitro.
Elucidar los mecanismos moleculares por los cuales la Cav-1 influye en la diferenciación de las BSCs en células multiciliadas (MCCs) y células secretoras (SCs).

Principales métodos:

Cultivo in vitro de células epiteliales de las vías respiratorias de ratón.
Evaluación de la expresión de Cav-1 en células madre basales (BSCs) y células multiciliadas (MCCs).
Análisis de la diferenciación y maduración de las MCCs en modelos deficientes de Cav-1.
Investigación de la actividad transcripcional del dominio intracelular de Notch (NICD).

Principales resultados:

La expresión de Caveolin-1 (Cav-1) es limitada en las células madre basales (BSCs) del epitelio de las vías respiratorias de ratón y se enriquece en las células multiciliadas (MCCs).
La deficiencia de Cav-1 acelera la diferenciación y maduración de las MCCs.
La regulación decreciente de Cav-1 impacta significativamente la actividad transcripcional del dominio intracelular de Notch (NICD).

Conclusiones:

La caveolina-1 (Cav-1) participa indirectamente en el programa transcripcional mediado por el dominio intracelular de Notch (NICD).
Cav-1 modula tanto la diferenciación de las células madre basales (BSCs) como la maduración de las células multiciliadas (MCCs) en el epitelio de las vías respiratorias.

Palabras clave:

Epitelio de las vías respiratorias Caveolina-1 Células multiciliadas Señalización Notch

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