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Transposición de la Técnica de Disolventes de Desarrollo Complementarios a la Cromatografía Flash: Una Estrategia de

Jason Fauquet¹, Adam Ellatiff¹, Claudio Palmieri¹

¹Laboratory of Therapeutic Chemistry and Pharmacognosy, Research Institute for Health Sciences and Technology, Research Institute for Bioscience, Faculty of Medicine, Pharmacy and Biomedical Sciences, University of Mons (UMONS), Mons, Belgium.

Journal of separation science

|February 16, 2026

Resumen

Este estudio adapta la técnica de Disolventes de Desarrollo Complementarios para la cromatografía flash, permitiendo la fracción eficiente de mezclas complejas. Este método acelera el descubrimiento de productos naturales al simplificar el análisis de muestras.

Área de la Ciencia:

Cromatografía
Química de Productos Naturales
Química Analítica

Sus antecedentes:

La cromatografía de capa fina de alta resolución (HPTLC) utiliza disolventes de desarrollo complementarios para mejorar la resolución.
La cromatografía flash en fase normal es una técnica común para el fraccionamiento de muestras.

Objetivo del estudio:

Adaptar la técnica de Disolventes de Desarrollo Complementarios para la cromatografía flash en fase normal.
Permitir el fraccionamiento sistemático y eficiente de extractos complejos de productos naturales.
Acelerar los flujos de trabajo de descubrimiento y desreplicación de productos naturales.

Principales métodos:

Adaptación de sistemas de disolventes de desarrollo de baja y alta polaridad para cromatografía flash.
Utilización de la Mezcla Universal HPTLC para pruebas de idoneidad del sistema y comparaciones entre técnicas.
Empleo de espectrometría de masas en tándem (MS/MS) para la identificación de compuestos y la confirmación del orden de elución.
Implementación de una estrategia de fraccionamiento por pasos isocráticos con siete ventanas de polaridad por gradiente.

Principales resultados:

Demostró la adaptación exitosa de la técnica de Disolventes de Desarrollo Complementarios a la cromatografía flash.
Confirmó la jerarquía de retención impulsada por la polaridad conservada en las escalas de HPTLC y cromatografía flash.
Logró una capacidad de pico (Pc) efectiva de 16.41 para el sistema de alta polaridad, lo que indica un equilibrio de separación.
Validó límites de fraccionamiento robustos y reproducibles en una matriz botánica compleja (Sideritis scardica).

Conclusiones:

La técnica adaptada proporciona un fraccionamiento sistemático y escalable para mezclas complejas.
Redujo el número de muestras para análisis posteriores, acelerando los ciclos de descubrimiento.
Ofrece una herramienta poderosa para la investigación de productos naturales, mejorando la eficiencia y el alcance.

Palabras clave:

transposición HPTLC a flash mezcla universal HPTLC fraccionamiento por pasos isocráticos flujo de trabajo de cromatografía preparativa flujo de trabajo de metabolómica no dirigida

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