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Análisis transcriptómico específico de células y knockout revelan la función de las acuaporinas en los movimientos

Lei Ding1, Maxime J Laurent1, Sylvain Legay2

  • 1Louvain Institute of Biomolecular Science and Technology, UCLouvain, 1348, Louvain-la-Neuve, Belgium.

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PubMed
Resumen

Las células de guarda (GC) y las células subsidiarias (SC) del maíz muestran patrones de expresión génica distintos. La eliminación de ZmPIP1 en las GC mejora la apertura estomática, especialmente bajo estrés hídrico.

Palabras clave:
ARNseqacuaporinasknockout específico de célulascélulas de guardamaízanálisis filogenéticomovimiento estomáticocélulas subsidiarias

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Área de la Ciencia:

  • Biología Vegetal; Biología Molecular; Genética

Sus antecedentes:

  • El movimiento estomático de las gramíneas depende de una estructura única de cuatro células que involucra células de guarda (GC) y células subsidiarias (SC).
  • Los mecanismos moleculares que rigen las interacciones célula a célula dentro de esta estructura aún no se comprenden completamente.

Objetivo del estudio:

  • Investigar la expresión génica, incluidas las acuaporinas (AQP), en las GC y SC del maíz.
  • Elucidar las funciones de acuaporinas específicas, ZmPIP1, en la regulación estomática utilizando un sistema de knockout basado en CRISPR.

Principales métodos:

  • Secuenciación de ARN de GC y SC microdiseccionadas de la epidermis de maíz durante el día y la noche.
  • Knockout tisular específico de genes ZmPIP1 en GC o SC mediante CRISPR.
  • Análisis transcriptómico y evaluación fenotípica de mutantes knockout en diversas condiciones hídricas.

Principales resultados:

  • Se observaron diferencias transcriptómicas significativas entre las GC y las SC, con las SC enriquecidas en genes del metabolismo lipídico y las GC en genes relacionados con la fotosíntesis.
  • Varias acuaporinas (AQP) mostraron variaciones en la expresión espacial y temporal.
  • La eliminación de ZmPIP1 en las GC provocó un aumento de la apertura estomática, especialmente bajo déficit hídrico leve.

Conclusiones:

  • Existen perfiles transcriptómicos distintos entre las GC y SC del maíz, lo que ofrece información sobre la regulación estomática de las gramíneas.
  • Las acuaporinas ZmPIP1 desempeñan un papel crucial en la regulación del movimiento estomático en el maíz.