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CleaveScope─Una herramienta fácil para el análisis de especificidad de proteasas a partir de datos LC-MS/MS

Mihail Konstantinov¹, Ilya Toropygin¹

¹Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia.

Analytical chemistry

|February 18, 2026

Resumen

CleaveScope es una nueva herramienta de software para analizar la especificidad de proteasas a partir de datos LC-MS/MS. Automatiza el análisis de sitios de escisión y visualiza las preferencias de aminoácidos, lo que ayuda a comprender la actividad enzimática.

Sus antecedentes:

El análisis de la especificidad de proteasas es crucial para comprender la función de las enzimas.
Los métodos existentes para analizar la especificidad de proteasas pueden ser lentos y complejos.
Los datos proteómicos de alto rendimiento, como LC-MS/MS, ofrecen una fuente rica para el perfilado de especificidad.

Conclusiones:

CleaveScope ofrece una solución eficiente y fácil de usar para el análisis de la especificidad de proteasas.
El software facilita una comprensión más profunda de los mecanismos enzimáticos y el reconocimiento de sustratos.
Disponible gratuitamente en GitHub, CleaveScope promueve la accesibilidad en la investigación proteómica.

Palabras clave:

especificidad de proteasas LC-MS/MS análisis de sitios de escisión bioinformática proteómica

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