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El fosfato de trifenilo induce cambios coordinados en la metilación del ADN y la expresión génica en células

Logan S Germain¹, Benjamin P Ott², Charles C T Hindmarch^1,2

¹Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.

Environmental epigenetics

|February 18, 2026

Resumen

La exposición al fosfato de trifenilo (TPHP) altera la expresión génica y la metilación del ADN en células de trucha. Este estudio revela cambios epigenéticos relacionados con el TPHP

Área de la Ciencia:

Toxicología ambiental
Epigenética
Toxicidad del desarrollo

Sus antecedentes:

El fosfato de trifenilo (TPHP) es un contaminante ambiental generalizado.
La exposición a TPHP se asocia con diversos efectos toxicológicos, incluida la disrupción endocrina y metabólica.
Los mecanismos epigenéticos pueden subyacer a los diversos resultados tóxicos del TPHP.

Objetivo del estudio:

Investigar las respuestas transcripcionales y epigenéticas coordinadas a la exposición a TPHP en una línea celular inmortalizada de trucha steelhead (STE-137).
Identificar los eventos moleculares iniciadores relevantes para las vías de resultados adversos.

Principales métodos:

Exposición de células STE-137 a TPHP (0 o 80 μM) durante 24 horas.
Secuenciación de ARN (RNA-seq) para el perfilado transcripcional.
Secuenciación de bisulfito de genoma completo (WGBS) para el análisis del metiloma.

Principales resultados:

La exposición a TPHP alteró significativamente 1622 genes, enriquecidos en vías de replicación del ADN, reparación, ciclo celular, señalización endocrina y metabolismo lipídico.
El análisis de metilación diferencial identificó 382 regiones metiladas diferencialmente (DMR), predominantemente hipermetiladas dentro de los genes.
La integración de datos transcriptómicos y epigenómicos reveló nueve genes con cambios concurrentes en la expresión y metilación, incluidas alteraciones en reguladores epigenéticos.

Conclusiones:

La exposición a TPHP induce alteraciones transcripcionales y epigenéticas significativas en células acuáticas.
Las modificaciones epigenéticas, en particular los cambios en la metilación del ADN, son eventos moleculares clave en la toxicidad del TPHP.
Los puntos finales epigenéticos son cruciales para evaluar la toxicidad del desarrollo y comprender las vías de resultados adversos del TPHP.

Palabras clave:

metilación del ADN toxicidad del desarrollo epigenética toxicidad de retardantes de llama disrupción metabólica transcriptómica fosfato de trifenilo

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