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Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
Published on: August 28, 2014
Hidrogeles reticulados físicamente a base de alcohol polivinílico y gelatina para la modulación independiente de las
Chen Feng1, Meng Chen1, Da-Hui Qu1
1Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Abstract:
Mechanical properties of biomaterials constitute a key parameter in regulating cellular responses. However, isolating the mechanical influence on cell behavior remains challenging due to the interdependent changes in chemical composition (e.g., polymer chain concentration or type, cross-linking agent, etc.) when modifying a single variable. Herein, noncovalently cross-linked hydrogels comprising poly(vinyl alcohol) (PVA) and gelatin (GEL) with gradient mechanical strength are fabricated by controlling the total freezing-thawing repetitions applied to the PVA-GEL mixture, in which the mechanical properties of hydrogels are successfully decoupled from the chemical composition for cell adhesion studies. The L929 cells prefer the stiff PVA-GEL hydrogel surfaces over the soft ones, uncovering significant variations in cellular attachment and spreading patterns. This strategy may provide a basis for systematically investigating the contribution of specific cues to cellular adhesion processes.
