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Updated: Feb 20, 2026

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Programación de la biosíntesis de ácido hialurónico de bajo peso molecular en Bacillus amyloliquefaciens mediante

Qian Zhong^1,2, Zhen Li^1,2, Waibin Duan^1,2

¹College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, P. R. China.

ACS synthetic biology

|February 18, 2026

Abstract:

Dynamic metabolic regulation facilitates real-time sensing of pathway states and precise redirection of metabolic flux, thereby enhancing the biosynthesis of diverse value-added compounds. Here, we designed an autoinducible molecular switch in Bacillus amyloliquefaciens by integrating a Pantoea alhagi-derived PaSspB-ssrA* proteolytic system with the DegU-RapG-PhrG quorum-sensing (QS) module. The OFF module employs the novel PaSspB-ssrA* system for targeted degradation of ssrA*-tagged proteins with 86% efficiency, and the ON module utilizes a DegU-activated P_ispA promoter for cell density-dependent gene expression. To improve hyaluronic acid (HA) production and reduce byproduct accumulation, this system was applied by fusing ssrA* tags into the glycolysis-critical genes pfkA and fruA to attenuate glycolytic flux, together with QS-regulated coexpression of PaSspB and SthHL in engineered Δldh/ΔwbpA/ΔnagB strains, achieving 13.5 ± 0.19 g/L of low-molecular-weight HA (2.89 × 10⁴ Da). The autonomous, inducer-free platform enables cost-effective HA biosynthesis in B. amyloliquefaciens, with broad applicability for synthesizing high-value bioproducts.

Palabras clave:

interruptor molecular autoinducible Bacillus amyloliquefaciens regulación dinámica ácido hialurónico ingeniería metabólica regulación del flujo metabólico

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