Video Experimental Relacionado

Updated: Feb 20, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

Transformación del factor de crecimiento β1 hacia la baja de la expresión de la triptonil-ARNt sintasa en

Aaron K McDowell-Sanchez¹, Konstantin Tsoyi²

¹Department of Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Baylor College of Medicine, Houston, United States.

Advances in medical sciences

|February 18, 2026

Abstracto

Purpose:

The role of tryptophanyl-tRNA synthetase, also known as WARS, in lung fibroblasts is currently unknown. We aimed to study the effect of transforming growth factor-beta 1 (TGF-β1) on the expression of WARS and whether it regulates profibrotic responses in TGF-β1-activated human lung fibroblasts.

Materials And Methods:

We used MRC-5, a human lung fibroblast cell line, and primary human lung fibroblasts (HLFs) derived from control subjects. WARS expression was measured by enzyme-linked immunosorbent assay (ELISA) and Western blot. Profibrotic responses in TGF-β1-stimulated human lung fibroblasts were measured by Western blot, gel contraction assay, and real-time quantitative PCR (RT-qPCR).

Results:

We demonstrate that TGF-β1 potently downregulates WARS expression, both at the extracellular and intracellular levels, in MRC-5, and HLFs. Yin Yang 1 (YY1) transcription factor (TF) silencing ameliorates the inhibitory effect of TGF-β1 on WARS expression. Finally, we found that recombinant (r) WARS significantly inhibited fibronectin (FN) but had no effect on collagen1 (COL1) or alpha-smooth muscle actin (αSMA) expression in TGF-β1-induced HLFs.

Conclusions:

Our results demonstrate that TGF-β1 inhibits WARS expression via YY1, however, WARS treatment has a modest effect on regulating profibrotic responses in activated HLFs.

Palabras clave:

Fibrosis pulmonar WARS1 fibroblastos pulmonares factor de crecimiento beta

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