Video Experimental Relacionado

Updated: Feb 20, 2026

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

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Published on: May 21, 2019

Análisis funcional de TRY de Rosa roxburghii (RrTRY) involucrado en el desarrollo de espinas y la respuesta a la

Wentao Ma¹, Min Wang², Mingcao Wang²

¹Engineering Research Center of National Forestry and Grassland Administration for Rosa Roxburghii, Agricultural College, Guizhou University, Guiyang 550025, People's Republic of China; Guizhou Technical Innovation Center of Cili Industry, Guiyang 550025, PR China.

Plant science : an international journal of experimental plant biology

|February 18, 2026

Abstract:

The aerial organs of Rosa roxburghii Tratt (RR), an economically important fruit crop endemic to southwestern China, have a dense prickle covering that severely impedes horticultural practices and industrial processing. Given the conserved role of R3MYB family members in inhibiting trichome formation in Arabidopsis thaliana, we systematically identified four R3MYB genes in the RR genome based on their conserved motifs. Promoter analysis revealed the presence of hormone-related elements in the promoter regions of the four R3MYB genes. Transcriptome data and qRT-PCR analysis revealed specific expression of RrTRY in prickle-free tissue. Subcellular localization analysis showed that RrTRY is targeted to both the nucleus and plasma membrane. Heterologous expression of RrTRY in A.thaliana suppressed trichome initiation, and in silico promoter analysis identified a cytokinin (CTK)-responsive cis-element. Exogenous CTK application restored trichome formation in transgenic A. thaliana, promoting endogenous AtTRY expression but suppressing RrTRY transcripts. Similarly, CTK application significantly increased the prickle density on leaf veins and young stems and downregulated RrTRY expression in RR seedlings. Our results demonstrate that CTK promotes prickle formation by repressing the negative regulator RrTRY, providing crucial molecular insights into prickle development and a strategic foundation for breeding prickle-free cultivars.

Palabras clave:

R3MYB Rosa roxburghii Tratt RrTRY citocinina espina

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