Video Experimental Relacionado

Updated: Feb 20, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

El complejo Znf711-Phf8 funciona como un reóstato transcripcional esencial para el desarrollo de neutrófilos

Shuiyi Tan¹, Huihui Qian², Haihong Wang²

¹Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruĳin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; CNRS IRP (International research Project), Cancer, Aging and Hematology, Sino-French Research Center for Life Sciences and Genomics, Ruĳin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040.

|February 19, 2026

Resumen

El complejo Znf711-Phf8, que se pensaba que activaba la transcripción, en realidad reprime el desarrollo de neutrófilos. Este descubrimiento revela un nuevo mecanismo que controla la maduración de los neutrófilos.

Área de la Ciencia:

Hematología
Biología Molecular
Epigenética

Sus antecedentes:

La diferenciación de neutrófilos es un proceso complejo regulado por factores transcripcionales y epigenéticos.
La proteína 711 con dedos de zinc (Znf711) y la proteína 8 con dedos de PHD desmetilasa de histonas (Phf8) se caracterizaron previamente como activadores transcripcionales.

Objetivo del estudio:

Investigar el papel del complejo Znf711-Phf8 en la diferenciación de neutrófilos.
Elucidar el mecanismo por el cual Znf711 y Phf8 regulan la granulopoyesis.

Principales métodos:

El estudio implicó el análisis de la función de Znf711 y Phf8 en la maduración de neutrófilos.
Las técnicas probablemente incluyeron ensayos de biología molecular para evaluar interacciones proteicas, expresión génica y modificaciones epigenéticas.

Principales resultados:

El complejo Znf711-Phf8 funciona como un represor transcripcional, no como un activador, en el desarrollo de neutrófilos.
Znf711 secuestra Phf8, inhibiendo su reclutamiento a los promotores de genes diana.
La pérdida de Znf711 conduce al reclutamiento de Phf8 por Gfi1aa al promotor de c/ebpα, inhibiendo la transcripción de c/ebpα.
Existe un bucle de retroalimentación positiva donde C/ebpα activa la expresión de Znf711, manteniendo altos niveles de C/ebpα durante la diferenciación.

Conclusiones:

El complejo Znf711-Phf8 actúa como un reóstato transcripcional crítico en el desarrollo de neutrófilos.
Este hallazgo redefine el papel funcional del complejo Znf711-Phf8 en la granulopoyesis.

Palabras clave:

desarrollo de neutrófilos Znf711-Phf8 represión transcripcional granulopoyesis c/ebpα

Más Videos Relacionados

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

Published on: April 9, 2013

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

Videos de Conceptos Relacionados

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Master Transcription Regulators

Master Transcription Regulators

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...