Video Experimental Relacionado

Updated: Feb 20, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Discriminación electroquímica selectiva de isomiRs de 3' que difieren en dos nucleótidos

Julio Ojeda¹, Nicholas Bruno¹, Karen Cover²

¹University of Central Florida. Chemistry Department, Orlando, FL 32816, USA. Karin.chumbimunitorres@ucf.edu.

|February 19, 2026

Abstract:

MicroRNAs and isomiRs are promising biomarkers for the detection of diseases such as cancers, but distinguishing the highly similar microRNA sequences between these remains challenging. We report a five-strand four-way junction (5S-4WJ) electrochemical biosensor that combines universal reporter strands with target-specific probes for a modular, low-cost design. The system discriminates miR-146b from its isomiR (R + 2, +2 nt at 3' end) by tuning the probe length, introducing mismatches, and controlling the hybridization kinetics. This strategy yields sevenfold higher sensitivity for R + 2 while maintaining specificity at 10-50 nM. Compared to existing electrochemical biosensors, which struggle to or do not test targets with extra nts at either 5' or 3', the 5S-4WJ sensor enables precise isomiR detection, advancing nucleic acid diagnostics.

Palabras clave:

isomiR microARN biosensor electroquímico diagnóstico del cáncer análisis molecular detección de ácidos nucleicos

Más Videos Relacionados

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Videos de Conceptos Relacionados

Restriction Enzymes

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...