Video Experimental Relacionado
Updated: May 5, 2026
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
Published on: March 10, 2016
El polisacárido de ñame chino promueve el desarrollo del yeyuno, la defensa antioxidante y la inmunidad de la mucosa
Chuanyan Che1,2, Ya Yang1,2, Hong Hu1
1Anhui Provincial Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Chuzhou, China.
Introduction:
Chinese yam polysaccharide (CYP), a heteropolysaccharide composed of mannose, xylose, arabinose, glucose, and galactose, has been reported to exhibit immunomodulatory and antioxidant properties. However, its role in regulating intestinal development and mucosal barrier function remains incompletely understood. This study investigated the effects of dietary CYP supplementation on antioxidant status, jejunal morphology, digestive function, and mucosal immunity in weaned rats.
Methods:
Thirty specific-pathogen-free male Sprague-Dawley rats (initial body weight: 50.16 ± 0.50 g) were randomly assigned to three dietary groups (n = 10 per group) for a 28-day feeding trial: a control group fed a basal diet and two treatment groups fed the basal diet supplemented with 0.1% or 0.5% CYP. At the end of the experiment, blood and jejunal tissues were collected for biochemical, histomorphological, and molecular analyses.
Results:
Compared with the control group, supplementation with 0.5% CYP significantly reduced plasma malondialdehyde (MDA) concentrations (P < 0.05) and increased total antioxidant capacity (P < 0.01), which was associated with activation of the MAPK ERK1/2-Nrf2 signaling pathway. Both 0.1% and 0.5% CYP supplementation significantly increased jejunal villus height and the villus height-to-crypt depth ratio (P < 0.05), while reducing intraepithelial lymphocyte numbers (P < 0.05). Moreover, rats receiving 0.5% CYP exhibited significantly higher activities of lactase, sucrase, and maltase, along with increased jejunal immunoglobulin A (IgA) and β-defensin levels (P < 0.05 or P < 0.01). Additionally, CYP supplementation at both inclusion levels markedly upregulated the expression of Claudin-1 and Mucin-1 (P < 0.01).
Discussion:
Collectively, these findings demonstrate that dietary CYP enhances systemic antioxidant defense, promotes jejunal structural development, and strengthens intestinal mucosal barrier function in weaned rats.
Más Videos Relacionados
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
10:03Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
Published on: August 15, 2025
