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Utilización de fructanos por miembros de Gammaproteobacteria marinas que involucran proteínas similares a SusC/D

Marie-Katherin Zühlke^1,2, Alexandra Bahr^1,3, Daniel Bartosik^1,2

¹Institute of Pharmacy, University of Greifswald, Germany.

The ISME journal

|February 19, 2026

Resumen

Bacterias marinas como Pseudoalteromonas distincta degradan fructanos utilizando novedosos sistemas de proteínas. Este descubrimiento amplía nuestra comprensión del metabolismo de carbohidratos marinos y los roles de los complejos similares a SusC/D más allá del filo Bacteroidota.

Área de la Ciencia:

Microbiología
Biología Marina
Bioquímica

Sus antecedentes:

Los fructanos son comunes en entornos terrestres pero menos estudiados en ecosistemas marinos.
La bacteria antártica Pseudoalteromonas distincta muestra adaptación a sustratos a base de fructosa.

Objetivo del estudio:

Investigar los mecanismos de degradación de fructanos en Pseudoalteromonas distincta.
Explorar la presencia y función de los loci de utilización de fructanos (PUL) en Gammaproteobacteria marinas.

Principales métodos:

Análisis proteómico
Ensayos bioquímicos
Análisis de secuencias
Análisis estructural
Genómica comparativa

Principales resultados:

P. distincta utiliza un novedoso locus de utilización de polisacáridos (PUL) de fructano que contiene una enzima GH32, una proteína similar a SusD y un transportador dependiente de TonB (TBDT) similar a SusC.
El complejo similar a SusC/D facilita la captación de fructanos de tipo inulina, seguida de la degradación periplasmática por una GH32 exoactiva.
Los fructanos de tipo levana son degradados extracelularmente por una GH32 separada y con acción endo.
La genómica comparativa revela sistemas similares en otras Gammaproteobacteria marinas, lo que sugiere una distribución más amplia.

Conclusiones:

Los complejos similares a SusC/D no son exclusivos de Bacteroidota y están presentes en Gammaproteobacteria.
Los fructanos son un componente importante del acervo de glicanos marinos.
Comunidades microbianas marinas especializadas se dirigen y degradan los fructanos.

Palabras clave:

Glicosil hidrolasa familia 32 Inulina Levana Locus de utilización de polisacáridos Proteínas de unión a glicanos de superficie Transportadores dependientes de TonB

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