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MdNF-YB14, un factor de transcripción de manzano NF-Y, actúa como un regulador positivo de la tolerancia a la sequía

Xiang Gao^1,2, Hongtao Wang², Penghao Yuan²

¹College of Horticulture, Hebei Agricultural University, Baoding, 07100L, China.

Plant cell reports

|February 19, 2026

Abstracto

Key Message:

MdNF-YB14 plays a critical role in enhancing drought stress tolerance by activating ROS- scavenging and stress-responsive genes. The nuclear factor Y (NF-Y) plays critical roles in maintaining plant growth, development, and stress tolerance. However, systematic research on the NF-Y gene family in apple remains insufficient. Here, a total of 47 MdNF-Y genes were identified in the apple genome, comprising 11 MdNF-YAs, 25 MdNF-YBs, and 11 MdNF-YCs. Analysis of cis-elements revealed that 30 out of 47 apple NF-Y gene promoters harbored one or more drought-responsive cis-elements, suggesting the potential involvement of NF-Y genes in plant drought tolerance. Among the 39 differentially expressed genes, 14 NF-Y genes were highly expressed in response to drought based on RNA-seq data. Notably, MdNF-YB14 showed significantly higher expression after 4 days of drought stress as confirmed by qRT-PCR analysis. Heterologous overexpression of MdNF-YB14 in transgenic Arabidopsis plants resulted in a higher germination rate, fresh weight, and root length under PEG treatment when compared with the wild-type plants. Similarly, overexpression of MdNF-YB14 positively enhanced drought tolerance in apple, which was associated with improved growth due to reduced electrolyte leakage, malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and superoxide radicals (O₂^-). Furthermore, the transcript levels of genes related to reactive oxygen species (ROS) scavenging and stress response were also significantly up-regulated in MdNF-YB14 transgenic apple plants. Collectively, MdNF-YB14 plays a crucial role in enhancing drought tolerance and can serve as a promising candidate gene for improving plant drought tolerance through biotechnological approaches.

Palabras clave:

Manzano Tolerancia a la sequía Identificación de función Factor de transcripción NF-Y

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Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

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