Video Experimental Relacionado

Updated: Jun 18, 2026

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

PAN2 mantiene la homeostasis de la cola de poli(A) del ARNm y regula la traducción durante la espermiogénesis en

Xuan Wu¹, Yu-Ke Wu¹, Meng-Yan Jia¹

¹Life Sciences Institute, Zhejiang Key Laboratory of Precise Protection and Promotion of Fertility, Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.

Nature communications

|February 19, 2026

Abstract:

The elongation and shortening of poly(A) tails are the most common types of post-transcriptional mRNA regulation in eukaryotic cells. PAN2-PAN3 and CCR4-NOT complexes are the main cytoplasmic enzymes that trim the poly(A) tails of mRNAs. However, the in vivo function of PAN2-PAN3 in mammals remains unclear. Here, we found that the germline-specific deletion of Pan2 causes male infertility due to a step-8/9 spermatogenic arrest, while meiotic prophase proceeds normally. Using PAIso-seq2 and mass spectrometry analyses, we define stage-specific remodeling of poly(A) tails that is disrupted in Pan2-null round spermatids (RS). Ribo-lite reveals a global reduction in translation efficiency in Pan2-deficient RS, correlating with proteomic changes and impairing pathways for spermatid differentiation. Endogenous IP-MS identifies PAN2 association with PABPC1 and initiation factors (EIF4E, EIF4A1, EIF5A), whose protein levels decline upon Pan2 loss, indicating compromised stability of key translational machinery. Together, these findings highlight the important physiological functions of PAN2 in spermiogenesis and expand our understanding of the post-transcriptional regulation of mRNAs in specific physiological processes.

Palabras clave:

espermatogénesis cola de poli(A)traducción esterilidad masculina ARN mensajero biología molecular biología reproductiva genética

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