Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

15.2K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
15.2K
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

2.5K
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
2.5K
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

5.6K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
5.6K
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

53.9K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
53.9K
Transport Across the Golgi01:26

Transport Across the Golgi

6.3K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
6.3K
What are Second Messengers?01:12

What are Second Messengers?

91.1K
Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
91.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A PI(3,5)P<sub>2</sub>/CHMP4B axis on lysosomes is essential for microautophagic degradation of STING.

Nature communications·2026
Same author

Visualizing Newly Synthesized Proteins and Their Degradation Dynamics by Using Long-Wavelength-Emitting Fluorescent Dye-DBCO Conjugates.

Bioconjugate chemistry·2026
Same author

Microautophagy: current understanding of its molecular mechanisms and functions.

Autophagy reports·2026
Same author

Single-molecule imaging quantifies oncogenic KRAS dynamics for enhanced accuracy of therapeutic efficacy assessment.

iScience·2025
Same author

Microautophagy: definition, classification, and the complexity of the underlying mechanisms.

Autophagy·2025
Same author

Reelin Increases the Sphingomyelin Content of the Plasma Membrane and Affects the Surface Expression of GPI-Anchored Proteins in Hippocampal Neurons.

Journal of neurochemistry·2025

Video Experimental Relacionado

Updated: Feb 22, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
11:25

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Published on: October 4, 2017

7.2K

Señalización de la Inmunidad Innata STING desde el Golgi

Tomohiko Taguchi1

  • 1Graduate School of Life Sciences, Tohoku University, Sendai, Japan. tomohiko.taguchi.b8@tohoku.ac.jp.

Sub-cellular biochemistry
|February 20, 2026
PubMed
Resumen

La vía del (guanilato-nucleótido-guanilato monofosfato) (GMPc-AMP) sintasa (cGAS)-estimulador de genes de interferón (STING) impulsa la inflamación. La activación de STING implica el transporte de RE a Golgi, con desregulación vinculada al síndrome COPA.

Palabras clave:
ColesterolIRF3Señalización inmune innataPalmitoilación de proteínasSTINGTBK1La red trans-Golgi

Más Videos Relacionados

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
11:44

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Published on: September 18, 2014

9.3K
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.4K

Videos de Experimentos Relacionados

Last Updated: Feb 22, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
11:25

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Published on: October 4, 2017

7.2K
Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
11:44

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Published on: September 18, 2014

9.3K
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.4K

Área de la Ciencia:

  • Inmunología; Biología Celular; Biología Molecular

Sus antecedentes:

  • La vía cGAS-STING es un regulador clave de la inmunidad innata, que detecta el ADN bicatenario (dsDNA) citosólico para iniciar respuestas inflamatorias.; La activación implica la translocación de STING desde el retículo endoplasmático (RE) al aparato de Golgi, lo que conduce a la producción de interferón de tipo I a través de TBK1 e IRF3.; La desregulación del tráfico de membranas entre el RE y el Golgi se asocia con enfermedades autoinflamatorias como el síndrome COPA.

Objetivo del estudio:

  • Explorar la regulación de la activación de la vía cGAS-STING por la dinámica del tráfico de membranas.; Elucidar los mecanismos moleculares que rigen la activación de TBK1/IRF3 en la red trans-Golgi (TGN).

Principales métodos:

  • Investigación de la localización y dinámica de STING utilizando imágenes de células vivas.; Análisis de interacciones proteína-proteína en la TGN.; Utilización de modelos genéticos del síndrome COPA para estudiar defectos en el transporte ER-Golgi.

Principales resultados:

  • La translocación de STING del RE al Golgi es esencial para su activación.; Eventos específicos de tráfico de membranas en la TGN son cruciales para el reclutamiento y activación de TBK1/IRF3.; Las mutaciones que afectan el transporte ER-Golgi alteran la señalización de STING y contribuyen a la autoinflamación.

Conclusiones:

  • El tráfico de membranas entre el RE y el Golgi es un punto de control regulatorio crítico para la vía cGAS-STING.; La modulación de la señalización mediada por STING y las vías de tráfico de membranas asociadas tiene un potencial terapéutico para enfermedades inflamatorias y cáncer.