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Protocolo para modelar el entrenamiento de la inmunidad innata por exposición repetida a alumbre en ratones

Thais Boccia¹, Matheus Deroco Veloso da Silva², Victor Fattori³

¹Department of Surgery, Boston Children's Hospital - Harvard Medical School, Boston, MA 02115, USA.

|February 20, 2026

Abstract:

We present a protocol to model the effects of innate immune training and reprogramming through repeated alum exposure in mice, enabling the evaluation of immunoregulatory cell populations in both steady-state and inflammatory conditions. We describe steps for integrating adoptive cell transfer, in vivo antigen-specific immunization, and transplant models. We also detail procedures for in vitro stimulation of human peripheral blood mononuclear cells (PBMCs) to explore translational relevance. For complete details on the use and execution of this protocol, please refer to Boccia et al.¹ and Boccia et al.².

Palabras clave:

Cultivo celular Aislamiento celular Ensayos celulares Citometría de flujo Inmunología

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