Análisis de fosfoinositidas de alta resolución

Cells derived from diseased tissue and their related cell lines exhibit numerous metabolic changes, including variations in lipid composition and metabolism. Indeed, lipids are important biomarkers of various diseases and exhibit crucial signaling roles during disease states. However, lipids, especially low-abundant and transient lipids like phosphoinositides, are difficult to study due to a lack of sophisticated tools. Here, we describe a unique targeted lipidomics method that allows us to define and compare the phosphoinositide composition of diseased and healthy tissues as well as related cell lines.