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Updated: Feb 24, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

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Published on: January 11, 2017

Desarrollo de inhibidores covalentes de quinasas de histidina bacterianas

Patricia D Rodriguez¹, Conrad A Fihn², Manibarsha Goswami¹

¹Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota, 55455, USA. carlsone@umn.edu.

Chemical communications (Cambridge, England)

|February 23, 2026

Abstract:

Antivirulence therapies offer an alternative strategy against antimicrobial resistance. We synthesized 2-aminobenzothiazole sulfonyl fluoride analogs for covalent histidine kinase inhibition. In vitro and in cellulo assays also identified lead inhibitors for affinity-based probe development. However, alkyne incorporation reduced bioactivity, emphasizing the challenges of designing functional probes while preserving inhibitor potency.

Palabras clave:

quinasa de histidina inhibidores covalentes terapias antivirulencia resistencia a los antimicrobianos sondas de afinidad química medicinal microbiología descubrimiento de fármacos

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