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Updated: Feb 25, 2026

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

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Published on: October 18, 2024

Etiquetado selectivo de ARN mediante reacciones IEDDA basadas en tetrazina con modificaciones de adenosina alílica

Feng Ge^1,2, Li Liu^1,2, Liang Cheng^1,2

¹Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.

Chembiochem : a European journal of chemical biology

|February 23, 2026

Resumen

No abstract available in PubMed .

Palabras clave:

etiquetado de ARN nucleósidos modificados con alilo química bioortogonal demanda inversa de Diels-Alder cicloadición de tetrazina

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
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