Video Experimental Relacionado
Updated: Feb 25, 2026
Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines
Published on: March 17, 2023
Identificación de genes compartidos y específicos de la enfermedad relacionados con las mitocondrias en EPOC, CPNM y
Siyu Wu1,2, Zelin Chen1, Tongxinwei Sun1
1Department of Pulmonary and Critical Care Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Abstract:
Chronic obstructive pulmonary disease (COPD) and non-small-cell lung cancer (NSCLC) often coexist; here, the shared mitochondrial drivers were investigated. Serum from 30 subjects (seven controls, nine COPD, eight NSCLC, and six NSCLC with COPD) underwent RNA-seq, integrated with 1,136 MitoCarta 3.0-derived mitochondrial-related genes (MRGs). DESeq2 identified 25, 124, and 58 mitochondria-related differentially expressed genes (MR-DEGs) in COPD, NSCLC, and their comorbidity, respectively, with 15 and 58 overlapping genes in relevant pairs. SVM-RFE selected two biomarker sets (3-gene and 5-gene), showing excellent diagnostic performance via ROC (AUC 0.89-0.92) and accurate multivariate logistic regression models. GSEA highlighted immune-inflammatory and oxidative phosphorylation pathways; CIBERSORT revealed altered immune cell proportions (e.g., elevated monocytes in COPD) with biomarker-immune cell correlations. CTD linked BID/COX7A2 to NSCLC, and DGIdb identified metformin/ME-344 as potential drugs. These mitochondrial gene signatures, validated in blood as robust classifiers of COPD, NSCLC, and their overlap, simultaneously furnish diagnostic biomarkers and actionable therapeutic targets, underscoring the translational value of mitochondria-immune crosstalk.
