Video Experimental Relacionado
Updated: Feb 25, 2026
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
Published on: June 2, 2023
Un método de visión por computadora para evaluar los linfocitos infiltrantes de tumores y modelado multiparamétrico
Mateusz Bielecki1,2, Fang-I Lu3, Angeline Vo4
1Biological Sciences Platform, Sunnybrook Research Institute, 2075 Bayview Ave., Room TB 097, Toronto, ON M4N 3M5, Canada.
Background:
Neoadjuvant systemic therapy (NST) is often used to treat locally advanced breast cancer (BC) or patients with early-stage BC at high risk for micrometastatic spread. Pathological complete response (pCR) to NST in BC is associated with excellent prognostic outcomes; however, rates vary significantly. Tumor-infiltrating lymphocytes (TILs) are associated with NST response, suggesting potential as predictive biomarkers.
Objective:
To develop a computer vision approach to quantify spatial TIL parameters and a multiparametric machine learning (ML) model for predicting NST response.
Design:
Retrospective, single institution study of 411 BC patients, combining clinical and graph-level pre-treatment histopathology data to predict response to NST using ML.
Methods:
Pre-treatment core needle biopsies were prepared, stained with hematoxylin and eosin, and digitized into whole slide images. Convolutional neural networks were applied to segment and classify regions of invasive carcinoma and TILs. Spatial features were extracted based on the coordinates of the TILs within invasive regions, including metrics from Delaunay triangulation, Voronoi diagram analysis, and minimum spanning trees, as well as features capturing cell density and nuclear count. Clinicopathological features were incorporated to support multiparametric modeling. Multiple ML classification models were trained to predict pCR. Logistic regression, K-nearest neighbor, support vector, random forest, Gaussian Naïve Bayes, and extreme gradient boosting models were tested, and model performances were reported.
Results:
ML models using clinical and graph-based features achieved high predictive accuracy. The best performing graph feature model reached an area under the receiver operating characteristic curve (AUC) of 0.924. Ensemble models integrating clinical and graph features showed the highest performance, with an AUC of 0.955. Notably, for triple-negative BC, significant differences in predictive performance were demonstrated between clinical and graph feature models (p = 0.026) and between clinical and ensemble models (p = 0.006).
Conclusion:
Multiparametric modeling utilizing clinicopathological and graph features obtained from TILs is associated with pCR in BC patients treated with NST.
