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Updated: Jun 23, 2026
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
Exosomal miR-26a derivado de preadipocitos bovinos promueve la adipogénesis intramuscular al dirigirse directamente a
Yiyang Du1, Jia Guo1, Zijiao Zhao2
1Jilin Provincial International Cooperation Key Laboratory for Science and Technology Innovation of Special Animal and Plants, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Abstract:
Intramuscular fat (IMF) deposition is a critical determinant of beef quality, yet the regulatory role of preadipocyte-derived exosomes remains unclear. Exosomes (HC-Exo and CRSC-Exo) were isolated from preadipocytes of Haoyue Beef Cattle (HC) and Chinese Red Steppe Cattle (CRSC), breeds with distinct IMF contents. HC-Exo significantly enhanced preadipocyte proliferation and differentiation, increasing lipid droplet formation and triglyceride synthesis, alongside elevated expression levels of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer binding protein alpha (C/EBPα). MiRNA sequencing identified 580 differentially expressed miRNAs, with miR-26a twice as abundant in HC-Exo versus CRSC-Exo. Integrated bioinformatics analyses, including KEGG, GO, and dual-luciferase reporter assays, identified glycogen synthase kinase-3B (GSK3B) as a direct target of miR-26a. Functional validation demonstrated that miR-26a mimics enhanced adipogenesis by suppressing GSK3B expression, while GSK3B overexpression inhibited differentiation. These findings establish exosomal miR-26a as a novel regulator of intramuscular adipogenesis, providing molecular insights for improving beef quality.
