Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Genomic DNA in Prokaryotes

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

DNA as a Genetic Template

DNA as a Genetic Template

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Direct Cytosolic Delivery of Amphiphilic Framework Nucleic Acids for RNA Interference.

Angewandte Chemie (International ed. in English)·2026

Same author

Innate Immunity of Framework Nucleic Acids.

Accounts of chemical research·2026

Same author

A multiple-encrypted DNA device for secure communication.

Science advances·2026

Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026

Same author

Structure-Controlled Molecular Recognition and Charge Transport in Metallized DNA Nanosheets.

Journal of the American Chemical Society·2026

Same author

Author Correction: Atomically precise photothermal nanomachines.

Nature materials·2026

Search research articles

Video Experimental Relacionado

Updated: Feb 26, 2026

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Grabadora Digital Codificada en un Marco de ADN para la Discriminación Bacteriana

Chenghao Xi¹, Junheng Zhang², Lu Song¹

¹Renji Branch of National Center for Translational Medicine (Shanghai), Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

|February 25, 2026

Resumen

Desarrollamos una Grabadora Digital de Marco de ADN (DFDR) para la detección simultánea de múltiples secuencias de ADN. Este sistema ofrece grabación molecular reescribible y de alta capacidad para diagnóstico y biología sintética.

Palabras clave:

marco de ADN detección de bacterias biosensor análisis de ácidos nucleicos codificación de posición

Más Videos Relacionados

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Videos de Experimentos Relacionados

Last Updated: Feb 26, 2026

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Área de la Ciencia:

Biología Sintética
Diagnóstico Molecular
Ingeniería Biomolecular

Sus antecedentes:

La detección simultánea de múltiples señales biomoleculares es crucial para el diagnóstico y la monitorización ambiental.
Los métodos actuales enfrentan desafíos en la enumeración multicanal dentro de una sola unidad.

Objetivo del estudio:

Desarrollar un sistema novedoso para la grabación de señales moleculares multiplexadas y de alta resolución.
Permitir la discriminación específica del sitio de múltiples secuencias de ácidos nucleicos utilizando un reportero uniforme.

Principales métodos:

Desarrollo de un sistema de codificación posicional basado en marcos de ADN (Grabadora Digital de Marco de ADN - DFDR).
Funcionalización de un marco de ADN triangular con sondas ortogonales dirigidas a distintas secuencias de ADN.
Demostración de la grabación de señales secuencial y reescribible a través del control temporal de las entradas de hebras de ADN.

Principales resultados:

Una sola unidad DFDR resolvió 18 objetivos de ácidos nucleicos simultáneamente, superando a los sistemas convencionales en 6 veces.
Discriminación exitosa del ARNr 16S de patógenos respiratorios clínicamente relevantes.
Validación de la identificación bacteriana en muestras del mundo real de hospitales y agua de río.

Conclusiones:

El sistema DFDR proporciona una plataforma versátil para el seguimiento de alta resolución de información molecular multiplexada.
Esta tecnología tiene amplias implicaciones para la biología sintética, el diagnóstico y el almacenamiento de información.
Permite el diagnóstico preciso de coinfecciones y la monitorización ambiental.