Análisis Espectrométrico de Metábolitos, Lípidos y Proteínas Inducidos por el Ejercicio Mediante Muestreo con Máscara...

Physical exercise significantly influences human metabolism, and these changes can be reflected in exhaled breath. Although various breath analysis methods have been established to monitor exercise-induced physiological changes, they are typically limited to tracking a single class of biomarkers and thus pose a challenging task in providing comprehensive molecular information about exercise-related breath alterations. Herein, we developed a cold-mask device for monitoring physiological conditions and collecting human exhaled breath during pre- and postexercise states. Breath metabolites, lipids, and proteins were condensed from exhaled breath using the cold-mask and then analyzed using mass spectrometry-based multiomics analysis. Significant changes in metabolites, lipids, and proteins were observed in exhaled breath between pre- and postexercise states. The exercise-induced metabolic pathways were further elucidated based on the multiomics analysis. This study not only provides a promising wearable device for monitoring dynamic breath changes during exercise but also offers new insights into exercise-induced physiological processes.