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Aislamiento robusto de monocitos ovinos primarios para estudios de infección específicos del hospedador reservorio

Benjamin U Bauer¹, Bidiepta Saha¹, Thierry Cottineau¹

¹Institute of Immunology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald, Insel Riems, Germany.

Journal of microbiological methods

|February 25, 2026

Abstract:

No widely available ovine monocyte/macrophage cell system exists. We present an affinity magnetic separation method combining gradient separation and magnetic bead selection to isolate pure primary ovine monocytes from blood, yielding large quantities of viable primary macrophages suitable for microbiological infection studies using reservoir-specific host cells targeted by intracellular pathogens.

Palabras clave:

Clamidia Macrófagos derivados de monocitos PBMC Oveja Zoonosis Coxiella

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