Video Experimental Relacionado

Updated: Feb 28, 2026

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

Interacción sinérgica y compensatoria única entre la isoenzima β-1,3-glucosiltransferasa en la biosíntesis de

Jingyun Liu¹, Mengmeng Xu², Yuxi Guo¹

¹School of Biotechnology, Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China.

International journal of biological macromolecules

|February 25, 2026

Resumen

La biosíntesis de polisacáridos de Ganoderma lucidum (GLPS) involucra dos enzimas clave, GL20535 y GL24465. La co-sobreexpresión aumenta la producción de polisacáridos, mientras que el co-silenciamiento la reduce, revelando sus roles cooperativos.

Área de la Ciencia:

Micología
Bioquímica
Biología Molecular

Sus antecedentes:

La biosíntesis de polisacáridos de Ganoderma lucidum (GLPS) es crucial para los hongos medicinales y comestibles.
Dos isoenzimas de β-1,3-glucosiltransferasa, GL20535 y GL24465, participan en la producción de GLPS.
La función específica de GL24465 y su interacción con GL20535 siguen sin estar claras.

Objetivo del estudio:

Investigar los roles individuales y combinados de GL20535 y GL24465 en la biosíntesis de GLPS.
Elucidar el mecanismo de interacción entre estas dos isoenzimas.
Comprender sus implicaciones para el rendimiento y la estructura de los polisacáridos.

Principales métodos:

Se emplearon técnicas de silenciamiento y sobreexpresión génica de forma individual y combinada.
Se realizó un análisis cuantitativo de polisacáridos intracelulares (IPS) y extracelulares (EPS).
Se evaluó el análisis transcripcional de enzimas clave y la composición de monosacáridos.

Principales resultados:

La modulación individual de GL24465 tuvo un impacto mínimo en la producción de polisacáridos.
La co-sobreexpresión mejoró significativamente la acumulación de polisacáridos intracelulares (IPS) (9,92 %) y extracelulares (EPS) (89,15 %)
El co-silenciamiento redujo la biomasa celular (13,81 %), el grosor de la pared celular (50,25 %), los IPS (35,02 %) y los EPS (20,52 %)
La co-regulación alteró la composición de monosacáridos de GLPS y las proporciones de enlaces glucosídicos al afectar las enzimas de síntesis de donantes de azúcar.

Conclusiones:

GL20535 es una enzima principal en la producción de GLPS, y GL24465 proporciona soporte compensatorio.
Estas isoenzimas cooperan para mantener el rendimiento y la integridad estructural de los polisacáridos.
Los hallazgos ofrecen información sobre la cooperación de isoenzimas de glicosiltransferasa para la biofabricación de polisacáridos optimizados.

Palabras clave:

Ganoderma lucidum Síntesis de polisacáridos Β-1,3-glucosiltransferasa

Más Videos Relacionados

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

Videos de Conceptos Relacionados

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Combined Effects of Drugs: Synergism

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...

Phase II Reactions: Glucuronidation

Phase II Reactions: Glucuronidation

Glucuronidation, a pivotal phase II biotransformation process, involves the coupling of glucuronic acid to a drug or xenobiotic. Given its widespread occurrence and critical role in drug metabolism, it's considered the most crucial phase II reaction. It enhances the water solubility of substances, aiding their expulsion from the body. The driving force behind these reactions is a group of enzymes known as UDP-glucuronosyltransferases (UGTs). UGTs facilitate the transfer of a glucuronic acid...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...