Efectos de la restricción calórica sobre el envejecimiento, el rendimiento del aprendizaje y la transcripción en...

Along with increasing an animal's lifespan, calorie restriction (CR) is shown to improve an animal's cognition. To elucidate the molecular differences that accompany CR that may benefit cognition, sibling Aplysia californica were reared on either an ad-lib (AL) or CR diet. Siblings from each diet were trained in two behaviors, learning food is inedible (LFI) and habituation of the tail withdrawal reflex (TWR), at two time points along their lifespans: younger animals at training time 1 (TT1) and aged siblings at training time 2 (TT2). In analysis by diet, TT2 CR animals' learning performance was on par with their TT1 CR siblings in both paradigms, illustrating a maintenance of cognition in age. Meanwhile, TT2 AL animals performed worse than TT1 AL siblings in habituation but better in LFI, illustrating the lack of cognitive maintenance in age. RNA sequencing was performed on part of the buccal ganglia that houses many of the neurons involved in LFI. Gene expression results implied morphological changes occurring within the motor and interneurons of the buccal ganglia after learning in LFI. These neurons showed enrichment of protein kinase C binding (GO:0005080), cadherin binding (GO:0045296), and microtubule severing ATPase activity (GO:0008568) as well as neuroactive ligand-receptor interaction (ko04080) and valine, leucine and isoleucine biosynthesis (ko00290) all of which have been implicated to assist in memory consolidation and reconsolidation.