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Updated: Feb 27, 2026

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PScnv: Detección personalizada de CNV autorregularizada con un marco jerárquico multifásico

Xuwen Wang^1,2,3, Zhili Chang^2,3,4, Wansheng Lv⁴

¹The Comprehensive Breast Care Center, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710003, China.

Bioinformatics (Oxford, England)

|February 26, 2026

Abstracto

Motivation:

Accurate detection of copy number variations (CNVs) from targeted panel sequencing remains challenging due to limited genomic coverage and pronounced sample-specific biases. Existing normalization strategies, including baseline-cohort, matched-control, and single-sample approaches, often struggle to balance noise suppression with adaptability, leading to inconsistent performance across heterogeneous samples.

Results:

We present PScnv, a personalized self-normalizing framework for robust CNV detection from panel sequencing data. PScnv integrates a pre-built panel-of-normals (PoN) with sample-intrinsic stable chromosomes through ridge-regression normalization to generate individualized log2 ratio profiles with reduced systematic variation. CNVs are then identified using a hierarchical multi-phase segmentation pipeline incorporating z-score pre-partitioning, kernel-based correction, and circular binary segmentation. In 139 clinical tumor samples with orthogonal FISH validation at MET, ERBB2, and MTAP, PScnv showed improved accuracy and robustness over existing methods that do not require patient-matched normal samples, provided that a pre-built PoN cohort is available.

Availability:

Source code is available for academic use at https://github.com/lvws/PScnv.

Supplementary Information:

Supplementary data are available at Bioinformatics online.

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